A chromogenic PLC substrate
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p-Nitrophenylphosphorylcholine

Item No. 21042

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-[[hydroxy(4-nitrophenoxy)phosphinyl]oxy]-N,N,N-trimethyl-ethanaminium, inner salt
CAS Number
21064-69-7
Synonyms
  • 4-Nitrophenylphosphorylcholine
  • O-(4-Nitrophenylphosphoryl)choline
  • para-Nitrophenylphosphorylcholine
  • 4-NPPC
  • p-NPPC
  • para-NPPC
Molecular Formula
C11H17N2O6P
Formula Weight
304.2
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 10 mg/mlEthanol: 10 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
217, 286 nm
SMILES
C[N+](C)(C)CCOP(OC1=CC=C([N+]([O-])=O)C=C1)([O-])=O
InChi Code
InChI=1S/C11H17N2O6P/c1-13(2,3)8-9-18-20(16,17)19-11-6-4-10(5-7-11)12(14)15/h4-7H,8-9H2,1-3H3
InChi Key
NAIXASFEPQPICN-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 mg

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    Product Description

    p-Nitrophenylphosphorylcholine is a chromogenic substrate that is used to measure phospholipase C (PLC) activity.1,2,3,4 Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Berka, R.M., Gray, G.L., and Vasil, M.L. Studies of phospholipase C (heat-labile hemolysin) in Pseudomonas aeruginosa. Infect. Immun. 34(3), 1071-1074 (1981).

    2. Fatmawati, N.N., Sakaguchi, Y., Suzuki, T., et al. Phospholipase C produced by Clostridium botulinum types C and D: comparison of gene, enzymatic, and biological activities with those of Clostridium perfringens alpha-toxin. Acta Med. Okayama. 67(1), 9-18 (2013).

    3. Kurioka, S. Sysnthesis of p-nitrophenylphosphorylcholine and the hydrolysis with phospholipase C. J. Biochem. 63(5), 678-680 (1968).

    4. Vanha-Perttula, T., and Kasurinen, J. A secretory phospholipase C-like enzyme in the bovine epididymal caput. FEBS J. 203(1), 69-72 (1986).