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2,6-Dibromoquinone-4-chloroimide is a colorimetric dye for the detection of phenolic compounds.1 It produces an indigo dye upon reacting with phenolic compounds at a pH of approximately 9.4. 2,6-Dibromoquinone-4-chloroimide has been used as a Gibbs reagent for the colorimetric detection of phenolic compounds. It has also been used in chromogenic reactions for the quantitative colorimetric detection of aflatoxins, causing colorless aflatoxins to become green with an absorption band at 673 nm.2
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1. Determination of phenol and structurally related compounds by Gibbs method. Anal. Chem. 20(12), 1191-1196 (1948).
2. Antibody-