A colorimetric dye for the detection of phenolic compounds
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2,6-Dibromoquinone-4-chloroimide

Item No. 21056

Technical Information
Formal Name
2,6-dibromo-4-(chloroimino)-2,5-cyclohexadien-1-one
CAS Number
537-45-1
Synonyms
  • NSC 528
Molecular Formula
C6H2Br2ClNO
Formula Weight
Purity
≥95%
A crystalline solid
DMF: 30 mg/mlDMF:PBS (pH 7.2); (1:7): 0.1 mg/mlDMSO: 25 mg/mlEthanol: 1 mg/ml
λmax
317 nm
SMILES
O=C(C(Br)=C/1)C(Br)=CC1=N\Cl
InChi Code
InChI=1S/C6H2Br2ClNO/c7-4-1-3(10-9)2-5(8)6(4)11/h1-2H
InChi Key
JYWKEVKEKOTYEX-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    2,6-Dibromoquinone-4-chloroimide is a colorimetric dye for the detection of phenolic compounds.1 It produces an indigo dye upon reacting with phenolic compounds at a pH of approximately 9.4. 2,6-Dibromoquinone-4-chloroimide has been used as a Gibbs reagent for the colorimetric detection of phenolic compounds. It has also been used in chromogenic reactions for the quantitative colorimetric detection of aflatoxins, causing colorless aflatoxins to become green with an absorption band at 673 nm.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ettinger, M.B., and Ruchhoft, C.C. Determination of phenol and structurally related compounds by Gibbs method. Anal. Chem. 20(12), 1191-1196 (1948).

    2. Du, B., Su, X., Yang, K., et alAntibody-free colorimetric detection of total aflatoxins in rice based on a simple two-step chromogenic reaction. Anal. Chem. 88(7), 3775-3780 (2016).