For immunochemical detection of diglycyl-lysine
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Diglycyl-Lysine Monoclonal Antibody (Clone GX41)

Item No. 21096

Technical Information
Immunogen
Diglycine-modified histone peptide
Clone Designation
GX41
100 µg of Protein G-purified monoclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide
Host
Mouse
Isotype
IgG1
Applications
IP and WB
Cross Reactivity
(+) Diglycyl-modified lysine(-) Unmodified lysine
Species Reactivity
(Independent) Species
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

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    Product Description

    Diglycyl-lysine is a ubiquitin remnant-containing peptide.1,2 Diglycyl-lysine is formed during protein digestion with trypsin, which cleaves the arginine residue of ubiquitin, leaving diglycyl conjugated to lysine in protein digests.2 It has been used as a marker of prior ubiquitin conjugation in trypsin protein digests.1 Cayman's Diglycyl-Lysine Monoclonal Antibody (Clone GX41) can be used for Western blot, and immunoprecipitation applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Xu, G., and Jaffrey, S.R. Proteomic identification of protein ubiquitination events. Biotechnol. Genet. Eng. Rev. 29(1), 73-109 (2013).

    2. Xu, G., Paige, J.S., and Jaffrey, S.R. Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling. Nat. Biotechnol. 28(8), 868-873 (2010).