A fluorogenic dye
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Tetramethylrhodamine ethyl ester (perchlorate)

Item No. 21426

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,6-bis(dimethylamino)-9-[2-(ethoxycarbonyl)phenyl]-xanthylium, perchlorate
CAS Number
115532-52-0
Synonyms
  • TMRE
  • TMR ethyl ester
Molecular Formula
C26H27N2O3 • ClO4
Formula Weight
515.0
Purity
≥98%
Emission
575 nm
Excitation
550 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: Slightly solublePBS (pH 7.2): Slightly soluble
λmax
226, 256, 352, 399, 550 nm
SMILES
O=C(OCC)C(C=CC=C1)=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4.O=Cl(=O)([O-])=O
InChi Code
InChI=1S/C26H27N2O3.ClHO4/c1-6-30-26(29)20-10-8-7-9-19(20)25-21-13-11-17(27(2)3)15-23(21)31-24-16-18(28(4)5)12-14-22(24)25;2-1(3,4)5/h7-16H,6H2,1-5H3;(H,2,3,4,5)/q+1;/p-1
InChi Key
NBAOBNBFGNQAEJ-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 mg

    $41

    Add

    10 mg

    $75

    Add

    25 mg

    $128

    Add

    50 mg

    $215

    Add
    Product Description

    Tetramethylrhodamine ethyl ester (TMRE) (perchlorate) is a non-cytotoxic cell-permeant fluorogenic dye most commonly used to assess mitochondrial function using live cell fluorescence microscopy and flow cytometry.1,2 It displays excitation/emission spectra of 550/575 nm, respectively. Due to the polarization of the mitochondrial membrane, TMRE is taken up into healthy mitochondria. However, when the membrane is depolarized, as in apoptosis, it is not taken up or is released from the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Farkas, D.L., Wei, M.-d., Febbroriello, P., et al. Simultaneous imaging of cell and mitochondrial membrane potentials. Biophys J. 56(6), 1053-1069 (1989).

    2. Sunaga, D., Tanno, M., Kuno, A., et al. Accelerated recovery of mitochondrial membrane potential by GSK-3β inactivation affords cardiomyocytes protection from oxidant-induced necrosis. PLoS One 9(11), e112529 (2014).