A fluorogenic dye
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Tetramethylrhodamine methyl ester (perchlorate)

Item No. 21437

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,6-bis(dimethylamino)-9-[2-(methoxycarbonyl)phenyl]-xanthylium, perchlorate
CAS Number
115532-50-8
Synonyms
  • TMRM
  • TMR methyl ester
Molecular Formula
C25H25N2O3 • ClO4
Formula Weight
500.9
Purity
≥98%
Emission
575 nm
Excitation
515, 555 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: slightly solublePBS (pH 7.2): slightly soluble
λmax
226, 255, 352, 550 nm
SMILES
O=C(OC)C(C=CC=C1)=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4.O=Cl(=O)([O-])=O
InChi Code
InChI=1S/C25H25N2O3.ClHO4/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5;2-1(3,4)5/h6-15H,1-5H3;(H,2,3,4,5)/q+1;/p-1
InChi Key
PFYWPQMAWCYNGW-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $55

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    10 mg

    $104

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    25 mg

    $190

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    50 mg

    $295

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    Product Description

    Tetramethylrhodamine methyl ester (TMRM) (perchlorate) is a non-cytotoxic cell-permeant fluorogenic dye most commonly used to assess mitochondrial function using live cell fluorescence microscopy and flow cytometry.1,2,3 It has two excitation peaks at 515 and 555 nm and an emission peak in the red-orange range (575 nm). Due to the polarization of the mitochondrial membrane, TMRM is taken up into healthy mitochondria. However, when the membrane is depolarized, as in apoptosis, it is not taken up or is released from the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Farkas, D.L., Wei, M.-d., Febbroriello, P., et al. Simultaneous imaging of cell and mitochondrial membrane potentials. Biophys J. 56(6), 1053-1069 (1989).

    2. Gandra, P.G., Nogueira, L., and Hogan, M.C. Mitochondrial activation at the onset of contractions in isolated myofibres during successive contractile periods. J. Physiol. 590(15), 3597-3609 (2012).

    3. Michea, L., Combs, C., Andrews, P., et al. Mitochondrial dysfunction is an early event in high-NaCl-induced apoptosis of mIMCD3 cells. Am. J. Physiol. Renal Physiol. 282(6), F981-F990 (2002).