A cell-permeable, potassium-sensitive fluorophore
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PBFI AM

Item No. 21602

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-1,3-benzenedicarboxylic acid, tetrakis[(acetyloxy)methyl] ester
CAS Number
124549-23-1
Synonyms
  • Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester
Molecular Formula
C58H62N2O24
Formula Weight
1171.1
Purity
≥95%
Emission
480-557 nm
Excitation
336 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: SolubleMethanol: Soluble
SMILES
COC1=CC2=C(OC(C3=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C3)=C2)C=C1N4CCOCCOCCN(C5=CC(OC(C6=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C6)=C7)=C7C=C5OC)CCOCCOCC4
InChi Code
InChI=1S/C58H62N2O24/c1-35(61)75-31-79-55(65)39-7-9-43(45(23-39)57(67)81-33-77-37(3)63)51-25-41-27-53(69-5)47(29-49(41)83-51)59-11-15-71-19-21-73-17-13-60(14-18-74-22-20-72-16-12-59)48-30-50-42(28-54(48)70-6)26-52(84-50)44-10-8-40(56(66)80-32-76-36(2
InChi Key
AUKRBWNSOWVABU-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

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    Product Description

    PBFI AM is a cell-permeable, potassium-sensitive fluorophore used to measure potassium changes in cells and intracellular compartments.1,2 As PBFI AM enters cells, it is hydrolyzed by intracellular esterases to produce PBFI. The affinity of PBFI for potassium is sodium-dependent (Kd = 44 and 5.1 mM with or without sodium, respectively).3 Absorption and emission maxima are 336 and 480-557 nm, respectively.4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Grieshaber, S., Swanson, J.A., and Hackstadt, T. Determination of the physical environment within the Chlamydia trachomatis inclusion using ion-selective ratiometric probes. Cell. Microbiol. 4(5), 273-283 (2002).

    2. Ljubkovic, M., Marinovic, J., Fuchs, A., et al. Targeted expression of Kir6.2 in mitochondria confers protection against hypoxic stress. J. Physiol. 577(Pt 1), 17-29 (2006).

    3. Halperin, S.J., and Lynch, J.P. Effects of salinity on cytosolic Na+ and K+ in root hairs of Arabidopsis thaliana: In vivo measurements using the fluorescent dyes SBFI and PBFI. J. Exp. Bot. 54(390), 2035-2043 (2003).

    4. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).

    5. Jezek, P., Mahdi, F., and Garlid, K.D. Reconstitution of the beef heart and rat liver mitochondrial K+/H+ (Na+/H+) antiporter. Quantitation of K+ transport with the novel fluorescent probe, PBFI. The Journal of Biological Chemisty 265(18), 10522-10526 (1990).