A fluorometric substrate for ALDH1A1 and chymotrypsin
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Resorufin Acetate

Item No. 21686

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-(acetyloxy)-3H-phenoxazin-3-one
CAS Number
1152-14-3
Synonyms
  • O-acetyl Resorufin
Molecular Formula
C14H9NO4
Formula Weight
255.2
Purity
≥98%
Emission
580 nm
Excitation
570 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMF:PBS (pH 7.2); (1:7): 0.12 mg/mlDMSO: 5 mg/mlEthanol: 0.1 mg/ml
λmax
214, 246, 345, 435 nm
SMILES
O=C1C=CC2=NC(C=CC(OC(C)=O)=C3)=C3OC2=C1
InChi Code
InChI=1S/C14H9NO4/c1-8(16)18-10-3-5-12-14(7-10)19-13-6-9(17)2-4-11(13)15-12/h2-7H,1H3
InChi Key
UJWKHDKBOVPINX-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 mg

    $36

    Add

    10 mg

    $68

    Add

    25 mg

    $153

    Add

    50 mg

    $268

    Add
    Product Description

    Resorufin acetate is a fluorometric probe that acts as a substrate for cytosolic aldehyde dehydrogenase (ALDH1A1) esterase activity.1 It is also a useful substrate for chymotrypsin.2 Upon enzymatic cleavage, the resorufin anion displays red fluorescence (ex/em maxima = 570/580 nm, respectively).3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kitson, T.M., and Kitson, K.E. Studies of the esterase activity of cytosolic aldehyde dehydrogenase with resorufin acetate as substrate. Biochem J. 322(Pt. 3), 701-708 (1997).

    2. Kitson, T.M. Studies on the chymotrypsin-catalysed hydrolysis of resorufin acetate and resorufin bromoacetate. Biochim. Biophys. Acta 1385(1), 43-52 (1998).

    3. Hofmann, J., and Sernetz, M. Immobilized enzyme kinetics analyzed by flow-through microfluorimetry: Resorufin-β-D-galactopyranoside as a new fluorogenic substrate for β-galactosidase. Anal. Chim. Acta 163, 67-72 (1984).