A high-affinity non-covalent extrinsic fluorescent dye
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bis-ANS (potassium salt)

Item No. 21820

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4,4'-bis(phenylamino)-[1,1'-binaphthalene]-5,5'-disulfonic acid, dipotassium salt
CAS Number
65664-81-5
Synonyms
  • 4,4'-Dianilino-1,1'-binaphthyl-5,5'-disulfonic Acid
Molecular Formula
C32H22N2O6S2 • 2K
Formula Weight
672.9
Purity
≥95%
Emission
523 (free), 496 (bound) nm
Excitation
390 (free), 484 (bound) nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: Slightly solublePBS (pH 7.2): 5 mg/ml
λmax
222, 275, 395 nm
SMILES
[O-]S(C1=CC=CC2=C1C(NC3=CC=CC=C3)=CC=C2C4=C(C=CC=C5S([O-])(=O)=O)C5=C(NC6=CC=CC=C6)C=C4)(=O)=O.[K+].[K+]
InChi Code
InChI=1S/C32H24N2O6S2.2K/c35-41(36,37)29-15-7-13-25-23(17-19-27(31(25)29)33-21-9-3-1-4-10-21)24-18-20-28(34-22-11-5-2-6-12-22)32-26(24)14-8-16-30(32)42(38,39)40;;/h1-20,33-34H,(H,35,36,37)(H,38,39,40);;/q;2*+1/p-2
InChi Key
VWLWTJHKQHRTNC-UHFFFAOYSA-L
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $38

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    5 mg

    $152

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    10 mg

    $209

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    Product Description

    bis-ANS is a high-affinity non-covalent extrinsic fluorescent dye used to analyze protein conformation.1 Its predominant interaction with proteins is through its hydrophobic phenyl and naphthyl rings.2 bis-ANS has an excitation maxima of 390 nm.3 It has an emission maximum of 523 nm when free in solution but undergoes a blue shift with an increase in fluorescence intensity when bound to protein; for example, when bound to intestinal fatty acid binding protein (FAPB2) it has emission maxima of 484-496 nm. bis-ANS has been used to label mechanically damaged neurons in acute brain slices.4 It also potently inhibits microtubule assembly.5,6

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hawe, A., Sutter, M., and Jiskoot, W. Extrinsic fluorescent dyes as tools for protein characterization. Pharm. Res. 25(7), 1487-1499 (2008).

    2. Bothra, A., Bhattacharyya, A., Mukhopadhyay, C., et al. A fluorescence spectroscopic and molecular dynamics study of bis-ANS/protein interaction. J. Biomol. Struct. Dyn. 15(5), 959-966 (1998).

    3. Pastukhov, A.V., and Ropson, I.J. Fluorescent dyes as probes to study lipid-binding proteins. Proteins 53(3), 607-615 (2003).

    4. Mozes, E., Hunya, A., Toth, A., et al. A novel application of the fluorescent dye bis-ANS for labeling neurons in acute brain slices. Brain Res. Bull. 86(3-4), 217-221 (2011).

    5. Horowitz, P., Prasad, V., and Luduena, R.F. Bis(1,8-anilinonaphthalenesulfonate). A novel and potent inhibitor of microtubule assembly. The Journal of Biological Chemisty 259(23), 14647-14650 (1984).

    6. Mazumdar, M., Parrack, P.K., Mukhupadbhyay, K., et al. Bis-ANS as a specific inhibitor for microtubule-associated protein induced assembly of tubulin. Biochemistry 31(28), 6470-6474 (1992).