For immunochemical detection of HLA-DR
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HLA-DR Monoclonal Antibody (Clone L243)

Item No. 21827

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • HLA Class II Histocompatibility Antigen DR
  • MHC Class II Antigen DR
Immunogen
Human lymphoblastoid cell line (RPMI 8866)
Clone Designation
L243
Formulation (Request formulation change)
100 µg of protein G-purified IgG
Storage Buffer
PBS, pH 7.2, containing 50% glycerol, with 0.1% BSA, and 0.02% sodium azide
Host
Mouse
Isotype
IgG2a
Applications
FC, ICC, IF, IHC, IP, and WB
Species Reactivity
(+) Human HLA-DR(+) Canine HLA-DR(+) Non-human primate HLA-DR
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $374

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    Product Description

    HLA-DR is a MHC Class II cell surface receptor heterodimer composed of a 33-35 kDa α chain, a ~30 kDa β chain, and a 10-30 amino acid ligand.1 When the heterodimer is fully combined on the cell surface of an antigen-presenting cell, such as macrophages, B cells, and dendritic cells, they present that ligand primarily to CD-4+ T cells.2 This presentation coupled with the T cell response can stimulate or suppress an antibody response to that ligand.2 HLR-DRs have been linked to a number of autoimmune disorders such as rheumatoid arthritis, lupus, and psoriasis as well as diabetes, hepatitis, and sclerosis among a number of others.3,4,5,6,7,8,9 Cayman’s HLA-DR Monoclonal Antibody (Clone L243) detects HLA-DR with or without attached ligand. The predicated size of the HLA-DRα or HLA-DRβ subunits is ~29-30 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Brodsky, F.M., and Parham, P. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J. Immunol. 128(1), 129-135 (1982).

    2. Denzin, L.K., Hammond, C., and Cresswell, P. HLA-DM interactions with intermediates in HLA-DR maturation and a role for HLA-DM in stabilizing empty HLA-DR molecules. J. Exp. Med. 184(6), 2153-2165 (1996).

    3. Panayi, G.S., Wooley, P., and Batchelor, J.R. Genetic basis of rheumatoid disease: HLA antigens, disease manifestations, and toxic reactions to drugs. Br. Med. J. 2(6148), 1326-1328 (1978).

    4. Messner, R.P., De Horatius, R., and Ferrone, S. Lymphocytotoxic antibodies in systemic lupus erythematosus patients and their relatives: reactivity with the HLA antigenic molecular complex. Arthritis Rheum. 23(3), 265-272 (1980).

    5. Tsuji, K., Inouye, H., Nose, Y., et al. Further study on HLA-A, B, C, D, DR and haplotype antigen frequencies in psoriasis vulgaris. Acta. Derm. Venereol. Suppl. (Stockh.) 87, 107-108 (1979).

    6. Solow, H., Hidalgo, R., and Singal, D.P. Juvenile-onset diabetes HLA-A, -B, -C, and -DR alloantigens. Diabetes 28(1), 1-4 (1979).

    7. Mackay, I.R., and Tait, B.D. HLA associations with autoimmune-type chronic active hepatitis: identification of B8-DRw3 haplotype by family studies. Gastroenterology 79(1), 95-98 (1980).

    8. Luckey, D., Bastakoty, D., and Mangalam, A.K. Role of HLA class II genes in susceptibility and resistance to multiple sclerosis: Studies using HLA transgenic mice. J. Autoimmun. 37(2), 122-128 (2011).

    9. Gorodezky, C., Alaez, C., Murguía, A., et al. HLA and autoimmune diseases: Type 1 diabetes (T1D) as an example. Autoimmun. Rev. 5(3), 187-194 (2006).