A fluorogenic substrate for acid and alkaline phosphatases
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Naphthol AS-BI-Phosphate

Item No. 21845

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)-2-naphthalenecarboxamide
CAS Number
1919-91-1
Synonyms
  • Napthol AS-BI Phosphoric Acid
  • N-ASBI-P
Molecular Formula
C18H15BrNO6P
Formula Weight
452.2
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 20 mg/mlDMSO:PBS(pH 7.2) (1:3): 0.25 mg/mlEthanol: 2 mg/ml
λmax
242 nm
SMILES
BrC1=CC=C(C=C(OP(O)(O)=O)C(C(NC2=C(OC)C=CC=C2)=O)=C3)C3=C1
InChi Code
InChI=1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)
InChi Key
HUXIAXQSTATULQ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 mg

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    Product Description

    Naphthol AS-BI-phosphate is a fluorogenic substrate for acid and alkaline phosphatases.1,2 Naphthol AS-BI-phosphate is converted to naphthol AS-BI (N-ASBI) which displays excitation/emission spectra of 405/515 nm, respectively. N-ASBI fluorescence is a quantitative marker of acid and alkaline phosphatase activity.1,2 The concentration of human acid and alkaline phosphatases undergo pronounced changes in particular diseases, resulting in unusually high or low concentrations. Thus, acid and alkaline phosphatases are often used as clinical markers of disease.3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Vaughan, A., Guilbault, G.G., and Hackney, D. Fluorometric methods for analysis of acid and alkaline phosphatase. Anal. Chem. 43(6), 721-724 (1971).

    2. Janckila, A.J., Takahashi, K., Sun, S.Z., et al. Naphthol-ASBI phosphate as a preferred substrate for tartrate-resistant acid phosphatase isoform 5b. J.Bone Miner Res. 16(4), 788-793 (2001).

    3. Bull, H., Murray, P.G., Thomas, D., et al. Acid phosphatases. Mol. Pathol. 55, 65-72 (2002).

    4. Yam, L.T. Clinical significance of the human acid phosphatases. Am. J. Med. 56, 604-616 (1974).