A fluorogenic substrate of enteropeptidase
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Enteropeptidase Fluorogenic Substrate (trifluoroacetate salt)

Item No. 22187

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(3S,6S,9S,12S,15S)-3-acetamido-19-amino-6,9,12-tris(carboxymethyl)-4,7,10,13-tetraoxo-15-((2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl)carbamoyl)-5,8,11,14-tetraazanonadecanoic acid, trifluoroacetate salt
Molecular Formula
C34H40F3N7O16 • XCF3COOH
Formula Weight
859.7
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: 10 mg/mlPBS (pH 7.2): 2.5 mg/ml
λmax
234, 340 nm
SMILES
NCCCC[C@H](NC([C@H](CC(O)=O)NC([C@H](CC(O)=O)NC([C@H](CC(O)=O)NC([C@H](CC(O)=O)NC(C)=O)=O)=O)=O)=O)C(NC1=CC=C(C(C(F)(F)F)=CC(O2)=O)C2=C1)=O.OC(C(F)(F)F)=O
InChi Code
InChI=1S/C34H40F3N7O16.C2HF3O2/c1-14(45)39-19(10-24(46)47)30(56)42-21(12-26(50)51)32(58)44-22(13-27(52)53)33(59)43-20(11-25(48)49)31(57)41-18(4-2-3-7-38)29(55)40-15-5-6-16-17(34(35,36)37)9-28(54)60-23(16)8-15;3-2(4,5)1(6)7/h5-6,8-9,18-22H,2-4,7,10-13,38H2,1H3,(H,39,45)(H,40,55)(H,41,57)(H,42,56)(H,43,59)(H,44,58)(H,46,47)(H,48,49)(H,50,51)(H,52,53);(H,6,7)/t18-,19-,20-,21-,22-;/m0./s1
InChi Key
JKYYEGXSJPEGSE-QXJGFYJDSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 µg

    $49

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    1 mg

    $86

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    5 mg

    $329

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    Product Description

    Enteropeptidase fluorogenic substrate is a substrate for enteropeptidase that contains a 7-amino-4-trifluoromethylcoumarin (AFC) moiety. Enteropeptidase is a serine protease expressed in the proximal small intestine of higher animals that converts inactive trypsinogen to active trypsin by endoproteolytic cleavage.1,2 Enteropeptidase recognizes the highly specific amino acid sequence DDDDK on the fluorogenic substrate and cleaves after the lysine residue, releasing the AFC moiety. Enteropeptidase activity is quantified by fluorescent detection of AFC, which displays excitation/emission spectra of 400/505 nm.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Pepeliaev, S., Krahulec, J., Černý, Z., et al. High level expression of human enteropeptidase light chain in Pichia pastoris. J. Biotechnol. 156(1), 67-75 (2011).

    2. Choi, M.-G., Lee, S., Chung, H.-S., et al. A fluorogenic method for measuring enteropeptidase activity: Spectral shift in the emission of GD4K-conjugated 7-amino-4-methylcoumarin. BMB Rep. 44(7), 458-461 (2011).