A fluorogenic substrate of MMPs
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NFF-3 (trifluoroacetate salt)

Item No. 22188

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N2-[2-(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-arginyl-L-prolyl-L-lysyl-L-prolyl-L-valyl-L-α-glutamyl-L-norvalyl-L-tryptophyl-L-arginyl-N6-(2,4-dinitrophenyl)-L-lysinamide, trifluoroacetate salt
Molecular Formula
C78H110N22O20 • XCF3COOH
Formula Weight
1675.8
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 25 mg/mlDMSO: 30 mg/mlDMSO:PBS(pH 7.2) (1:3): 0.25 mg/mlEthanol: slightly soluble
λmax
219, 269, 291, 331 nm
SMILES
O=C([C@H](CC1=CNC2=C1C=CC=C2)NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H]3N(C([C@@H](NC([C@H]4N(C([C@H](CCCNC(N)=N)NC(CC5=CC(OC6=C5C=CC(OC)=C6)=O)=O)=O)CCC4)=O)CCCCN)=O)CCC3)=O)C(C)C)=O)CCC(O)=O)=O)CCC)=O)N[C@H](C(N[C@@H](CCCCNC7=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C7)C(N)=O)=O)CCCNC(N)=N.FC(F)(F)C(O)=O
InChi Code
InChI=1S/C78H110N22O20.C2HF3O2/c1-5-16-53(68(106)95-58(37-45-42-88-50-18-7-6-17-48(45)50)71(109)92-54(21-12-33-86-77(81)82)69(107)90-52(67(80)105)19-9-11-32-85-51-28-25-46(99(115)116)40-61(51)100(117)118)91-70(108)55(29-30-64(102)103)93-74(112)66(43(2)3)96-73(111)60-24-15-36-98(60)76(114)57(20-8-10-31-79)94-72(110)59-23-14-35-97(59)75(113)56(22-13-34-87-78(83)84)89-63(101)38-44-39-65(104)120-62-41-47(119-4)26-27-49(44)62;3-2(4,5)1(6)7/h6-7,17-18,25-28,39-43,52-60,66,85,88H,5,8-16,19-24,29-38,79H2,1-4H3,(H2,80,105)(H,89,101)(H,90,107)(H,91,108)(H,92,109)(H,93,112)(H,94,110)(H,95,106)(H,96,111)(H,102,103)(H4,81,82,86)(H4,83,84,87);(H,6,7)/t52-,53-,54-,55-,56-,57-,58-,59-,60-,66-;/m0./s1
InChi Key
RQAYJVJJNNJVIL-QFOZWALASA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

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    Product Description

    NFF-3 is a fluorogenic substrate of matrix metalloproteinases (MMPs).1 NFF-3 is hydrolyzed rapidly by MMP-3 (kcat/Km = 218,000 s-1*M-1) and slowly by MMP-9 (kcat/Km = 10,100 s-1*M-1) with no significant hydrolysis by MMP-1 or MMP-2.1 NFF-3 can be used to differentiate MMP-3 activity from that of other MMPs.1,2 

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Giricz, O., Lauer, J.L., and Fields, G.B. Comparison of Metalloproteinase protein and activity profiling. Anal. Biochem. 409(1), 37-45 (2011).