An inhibitor of the Mad2-Cdc20 interaction
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M2I-1

Item No. 22297

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-[[4-[bis(2-methylpropyl)amino]-3-nitrophenyl]methylene]dihydro-2-thioxo-4,6(1H,5H)-pyrimidinedione
CAS Number
312271-03-7
Synonyms
  • Mad2 Inhibitor-1
Molecular Formula
C19H24N4O4S
Formula Weight
404.5
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 25 mg/mlDMSO: 25 mg/mlEthanol: slightly soluble
λmax
229, 247, 272, 467 nm
SMILES
S=C1NC(/C(C(N1)=O)=C/C2=CC=C(N(CC(C)C)CC(C)C)C([N+]([O-])=O)=C2)=O
InChi Code
InChI=1S/C19H24N4O4S/c1-11(2)9-22(10-12(3)4)15-6-5-13(8-16(15)23(26)27)7-14-17(24)20-19(28)21-18(14)25/h5-8,11-12H,9-10H2,1-4H3,(H2,20,21,24,25,28)
InChi Key
BWEKPQUKWLNUKX-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

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    10 mg

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    25 mg

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    Product Description

    M2I-1 is an inhibitor of the protein-protein interaction between the spindle assembly checkpoint protein mitotic arrest deficient 2 (Mad2) and cell division cycle 20 (Cdc20), a coactivator of the anaphase-promoting complex/cyclosome (APC/C).1 It inhibits Mad2 binding to Cdc20111-138 when used at concentrations ranging from 6.25 to 100 μM. M2I-1 (25 μM) reduces increases in mitotic duration induced by paclitaxel (Item No. 10461) in HeLa cells. M2I-1 (20-120 μM) reduces 4-cell embryo and blastocyst formation in hydrogen peroxide-exposed mouse zygotes, as well as increases the rate of sex chromosome mosaicism in male mouse in vitro fertilization-derived embryos.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kastl, J., Braun, J., Prestel, A., et al. Mad2 inhibitor-1 (M2I-1): A small molecule protein-protein interaction inhibitor targeting the mitotic spindle assembly checkpoint. ACS Chem. Biol. 10(7), 1661-1666 (2015).

    2. Huang, Y., Ha, S., Li, Z., et al. CHK1-CENP B/MAD2 is associated with mild oxidative damage-induced sex chromosome aneuploidy of male mouse embryos during in vitro fertilization. Free Radic. Biol. Med. 137, 181-193 (2019).