Active • Host: E. coli • AA: 1-336 (full length) • Tags: N-terminal Trx, His, and S • MW: 52.71 kDa
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CdnP (Mycobacterium tuberculosis strain ATCC 25618/H37Rv recombinant)

Item No. 22809

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bifunctional Oligoribonuclease and PAP Phosphatase NrnA
  • 3'(2'),5'-Bisphosphate Nucleotidase
  • CnpB
  • Cyclic di-NMP Phosphodiesterase
  • PAP Phosphatase
  • 3'-Phosphoadenosine 5'-phosphate Phosphatase
  • Rv2837c
Purity
≥70% estimated by SDS-PAGE
Source
Active recombinant M. tuberculosis strain ATCC 25618/H37Rv N-terminal Trx-, His-, and S-tagged CdnP expressed in E. coli
Amino Acids
1-336 (full length)
MW
52.71 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 8.0, with 150 mM sodium chloride
UniProt Accession №
P71615
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $436

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    Cayman Chemical
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    Product Description

    Cyclic dinucleotide phosphodiesterase (CdnP) is a soluble phosphodiesterase involved in regulating cyclic dinucleotide signaling during intracellular infections of M. tuberculosis.1 It is composed of an N-terminal Asp-His-His (DHH) domain and a C-terminal DHH-associated (DHHA1) domain that catalyze the hydrolysis of cyclic dinucleotides. CdnP hydrolyzes a variety of cyclic dinucleotides, including cyclic di-AMP (Item No. 17753), cyclic di-GMP (Item No. 17144), and 2′3′-cGAMP (Item No. 19887), which decreases their recognition by the stimulator of interferon genes (STING), a key mediator in the host innate immune response.2,3,4 Knockout of the gene encoding CdnP, CnpB, increases the production of IFN-β in isolated mouse bone marrow-derived macrophages (BMDMs) infected with CnpB-/- M. tuberculosis, as well as decreases pulmonary and splenic bacterial burden and increases survival in CnpB-/- M. tuberculosis-infected mice.5 Cayman’s CdnP (Mycobacterium tuberculosis strain ATCC 25618H37Rv recombinant) protein contains N-terminal Trx- and His-tags followed by a thrombin cleavage site and an S-tag followed by an enterokinase cleavage site.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. He, Q., Wang, F., Liu, S., et al. Structural and biochemical insight into the mechanism of Rv2837c from Mycobacterium tuberculosis as a c-di-NMP phosphodiesterase. The Journal of Biological Chemisty 291(27), 14386-14387 (2016).

    2. Cheng, S.S., Chung, M.J., Lin, C.Y., et al. Phytochemicals from Cunninghamia konishii Hayata act as antifungal agents. J. Agric. Food Chem. 60(1), 124-128 (2012).

    3. Dey, R.J., Dey, B., Zheng, Y., et al. Inhibition of innate immune cytosolic surveillance by an M. tuberculosis phosphodiesterase. Nat. Chem. Biol. 13(2), 210-217 (2017).

    4. Jeong, H.U., Kwon, S.S., Kong, T.Y., et al. Inhibitory effects of cedrol, β-cedrene, and thujopsene on cytochrome P450 enzyme activities in human liver microsomes. J. Toxicol. Environ. Health A 77(22-24), 1522-1532 (2014).

    5. Yang, J., Bai, Y., Zhang, Y., et al. Deletion of the cyclic di-AMP phosphodiesterase gene (cnpB) in Mycobacterium tuberculosis leads to reduced virulence in a mouse model of infection. Mol. Microbiol. 93(1), 65-79 (2014).