Active • Host: E. coli • AA: 2-543 (full length) • Tag: N-terminal His • MW: 61 kDa
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cGAS (human, recombinant)

Item No. 22810

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • C6orf150
  • 2’3’-cGAMP Synthase
  • cGAMP Synthase
  • h-cGAS
  • Cyclic GMP-AMP Synthase
  • EC: 2.7.7.86
  • Mab-21 domain containing protein 1
  • MB21D1
Purity
≥90% estimated by SDS-PAGE
Source
Active recombinant N-terminal His-tagged protein expressed in E. coli
Amino Acids
2-522 (full length)
MW
61 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 8.0, with 300 mM sodium chloride, and 10% glycerol
UniProt Accession №
Q8N884
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $677

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    Product Description

    Cyclic GMP-AMP (cGAMP) synthase (cGAS) is a nucleotidyltransferase located in the cytosol that acts as a cytosolic DNA sensor to detect foreign DNA from microbial pathogens as part of the innate immune response.1,2 Upon binding to cytosolic DNA, cGAS produces the cyclic dinucleotide second messenger cGAMP, which activates stimulator of interferon genes (STING), leading to activation of the type I interferon (IFN) pathway.1,2,3 In vitro, fibroblasts, macrophages, and dendritic cells isolated from cGAS knockout (cGAS-/-) mice do not produce type I IFNs following DNA transfection or DNA virus infection.4 Similarly, cells containing a frame-shift mutation in the cGAS locus fail to mount an immune response to HIV and other retroviruses.5 In vivo, cGAS-/- mice infected with herpes simplex virus 1 (HSV-1) have lower levels of IFN-α and IFN-β, shorter survival times, and higher post-mortem levels of HSV-1 in the brain.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sun, L., Wu, J., Du, F., et al. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science 339(6121), 786-791 (2013).

    2. Wu, J., Sun, L., Chen, X., et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science 339(6121), 826-830 (2013).

    3. Ablasser, A., Goldeck, M., Cavlar, T., et al. cGAS produces a 2'-5'-linked cyclic dinucleotide second messenger that activates STING. Nature 498(7454), 380-384 (2013).

    4. Li, X.-D., Wu, J., Gao, D., et al. Pivotal roles of cGAS-cGAMP signaling in antiviral defense and immune adjuvant effects. Science 341(6152), 1390-1394 (2013).

    5. Gao, D., Wu, J., Wu, Y.-T., et al. Cyclic GMP-AMP synthase is an innate immune sensor of HIV and other retroviruses. Science 341(6148), 903-906 (2013).

    Product Citations

    Fréreux, C., Karam, J.A.Q., Howley, B.V., et al. PCBP1 binding to single-stranded poly-cytosine motifs enhances cGAS sensing and impairs breast cancer development. Commun. Biol. 9(1), 179 (2026).

    Fréreux, C., and Howe, P.H. ELISA-based enzyme kinetics assay for measuring cGAS activity. Bio. Protoc. 16(7), e5655 (2026).

    Hao, X., Zhao, B., Towers, M., et al. TXNRD1 drives the innate immune response in senescent cells with implications for age-associated inflammation. Nat. Aging 4(2), 185-197 (2024).

    Valentin, R., Wong, C., Alharbi, A.S., et al. Sequence-dependent inhibition of cGAS and TLR9 DNA sensing by 2'-O-methyl gapmer oligonucleotides. Nucleic Acids Res. 49(11), 6082-6099 (2021).

    Fukuda, S., Narendran, S., Varshney, A., et al. Alu complementary DNA is enriched in atrophic macular degeneration and triggers retinal pigmented epithelium toxicity via cytosolic innate immunity. Sci. Adv. 7(40), eabj3658 (2021).

    Liu, D., Lum, K.K., Treen, N., et al. IFI16 phase separation via multi-phosphorylation drives innate immune signaling. Nucleic Acids Res. 51(13), 6819-6840 (2023).