Human recombinant enzyme
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IRF3 (human, recombinant)

Item No. 22811

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Interferon Regulatory Factor 3
Purity
≥85% as estimated by SDS-PAGE
Source
N-terminally Histidine-tagged human IRF3 protein (full length) expressed in E. coli
Amino Acids
1-427 (full length)
MW
49.6 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 8.0, with 150 mM sodium chloride and 10% glycerol
UniProt Accession №
Q14653
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $534

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    Product Description

    Interferon regulatory factor 3 (IRF3) is a member of the IRF family. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection, functioning as a molecular switch for antiviral activity.1,2,3,4,5 Double-stranded RNA generated during a viral infection leads to IRF3 activation by serine/threonine phosphorylation by TBK1 or IKBKE kinases, which induces a conformational change leading to its dimerization, nuclear localization, and association with CREBBP.1,2,3,6 The complex, known as DRAF1, activates transcription interferons alpha and beta, as well as other interferon-induced genes.1,6 These genes play a critical role in type 1 interferon (IFN)-dependent immune response.5 A recent study shows phosphorylation of innate immune adaptor proteins MAVS, STING, and TRIF recruits and specifies IRF3 phosphorylation and activation by the serine/threonine-protein kinase TBK1, thereby inducing the production of type-1 interferons.2,4,7

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Shu, C., Sankaran, B., Chaton, C.T., et al. Structural insights into the functions of TBK1 in innate antimicrobial immunity. Structure 21(7), 1137-1148 (2013).

    2. Huang, J., Liu, T., Xu, L.G., et al. SIKE is an IKKε/TBK1-associated suppressor of TLR3- and virus-triggered IRF-3 activation pathways. EMBO J. 24(23), 4018-4028 (2005).

    3. tenOever, B.R., Servant, M.J., Grandvaux, N., et al. Recognition of the measles virus nucleocapsid as a mechanism of IRF-3 activation. J. Virol. 76(8), 3659-3669 (2002).

    4. Xu, L.G., Wang, Y.Y., Han, K.J., et al. VISA is an adapter protein required for virus-triggered IFN-β signaling. Mol. Cell. 19(6), 727-740 (2005).

    5. Peteranderl, C., and Herold, S. The impact of the interferon/TNF-related apoptosis-inducing ligand signaling axis on disease progression in respiratory viral infection and beyond. Front. Immunol. 8:13, (2017).

    6. Gu, L., Fullam, A., Brennan, R., et al. Human DEAD box helicase 3 couples IκB kinase ε to interferon regulatory factor 3 activation. Mol. Cell. Biol. 33(10), 2004-2015 (2013).

    7. Liu, S., Cai, X., Wu, J., et al. Phosphorylation of innate immune adaptor proteins MAVS, STING, and TRIF induces IRF3 activation. Science 347(6227), aaa2630 (2015).