A tropic hormone
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Thyrotropin-Releasing Hormone

Item No. 22917

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-oxo-L-prolyl-L-histidyl-L-prolinamide
CAS Number
24305-27-9
Synonyms
  • pGlu-His-Pro amide
  • Thyroliberin
  • Thyrotropin-Releasing Factor
  • TRF
  • TRH
  • TSH-releasing Hormone
Molecular Formula
C16H22N6O4
Formula Weight
362.4
Purity
≥98%
Formulation
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: 30 mg/mlPBS (pH 7.2): 10 mg/ml
SMILES
O=C(N1CCC[C@H]1C(N)=O)[C@H](CC2=CNC=N2)NC([C@@H]3CCC(N3)=O)=O
InChi Code
InChI=1S/C16H22N6O4/c17-14(24)12-2-1-5-22(12)16(26)11(6-9-7-18-8-19-9)21-15(25)10-3-4-13(23)20-10/h7-8,10-12H,1-6H2,(H2,17,24)(H,18,19)(H,20,23)(H,21,25)/t10-,11-,12-/m0/s1
InChi Key
XNSAINXGIQZQOO-SRVKXCTJSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    10 mg

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    Product Description

    Thyrotropin-releasing Hormone (TRH) is a tropic hormone that stimulates release of thyroid-stimulating hormone (TSH) and prolactin.1 It binds to the human TRH receptor (TRHR) expressed in CHO cells and to rat TRHR in rat brain membranes (IC50s = 25 and 198 nM, respectively).2,3 TRH (1 μM) decreases polyphosphoinositide and increases arachidonic and oleic acid diacylglycerol incorporation into lipids of GH3 rat pituitary tumor cells.4 In vitro, it dose-dependently increases TSH release from rat anterior pituitary slices and prolactin release from cultured anterior pituitary cells.5,6 In vivo, TRH (0.01 mg/kg) increases TSH and prolactin plasma levels in rats.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dettmar, P.W., Lynn, A.G., Metcalf, G., et al. The ability of RX 77368 - a stabilised analogue of TRH - to provoke the secretion of prolactin and TSH in vivo. Neuropeptides 3(1), 1-8 (1982).

    2. Yamada, M., Iwasaki, T., Satoh, T., et al. Activation of the thyrotropin-releasing hormone (TRH) receptor by a direct precursor of TRH, TRH-Gly. Neurosci. Lett. 196(1-2), 109-112 (1995).

    3. Bhargava, H.N., and Das, S. Evidence for opiate action at the brain receptors for thyrotropin-releasing hormone. Brain Res. 368(2), 262-267 (1986).

    4. Martin, T.F.J. Thyrotropin-releasing hormone rapidly activates the phosphodiester hydrolysis of polyphosphoinositides in GH3 pituitary cells. Evidence for the role of a polyphosphoinositide-specific phospholipase C in hormone action. The Journal of Biological Chemisty 258(24), 14816-14822 (1983).

    5. Iriuchijima, T., Michimata, T., Miyashita, K., et al. Thyroid hormones regulate the formation of inositol phosphate in response to thyrotropin-releasing hormone in rat anterior pituitaries. Neuropeptides 21(1), 49-53 (1992).

    6. Apfelbaum, M.E. Role of vasoactive intestinal peptide and 5-HT2 receptor subtype in serotonin stimulation of basal and thyrotropin-releasing-hormone-induced prolactin release in vitro from rat pituitary cells. Neuroendocrinology 67(1), 45-50 (1998).