Cyclic AMP TR-FRET Kit | Cayman Chemical
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Cyclic AMP TR-FRET Kit

Item № 23042
     1 ea $399.00 0.00

Pricing updated 2018-12-14. Prices are subject to change without notice.

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  • A time-resolved fluorescence energy transfer assay for the detection of cAMP in cell based assay systems
  • Reagents supplied to run up to 1,000 assays in 384 well plates OR 2,000 assays in 1536 well plates
  • Adenosine 3',5'-cyclic mononucleotide
  • Adenosine 3',5'-cyclic monophosphate

The TR-FRET cAMP Assay is designed to allow the user to detect and quantitatively measure the amount of cyclic AMP (cAMP) present in cell based assay systems. A europium (Eu) labeled monoclonal anti-cAMP antibody (Donor) is paired with a fluorescently labeled cAMP analog (Acceptor), and in the absence of any endogenous cAMP, will produce a time-resolved energy transfer signal at 665 nm when excited at 340 nm. If cAMP accumulates in the well due to the activation of cellular adenylyl cyclase, this cAMP inhibits the formation of the labeled Donor/Acceptor complex, decreasing the amount of energy transfer signal produced, with the overall drop in signal being inversely proportional to the amount of cAMP accumulated.

Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.

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Technical Information
  • Adenosine 3',5'-cyclic mononucleotide
  • Adenosine 3',5'-cyclic monophosphate

Warning - this product is not for human or veterinary use.

Shipping & Storage
Wet ice in continental US; may vary elsewhere
≥ 6 months
Downloads & Resources
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Download Kit Booklet

Download free InChI Key generation software

Additional Information

View the Cayman Structure Database for chemical structure definitions for many Cayman products

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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data pack, and GC-MS data.

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Ann Arbor, Michigan 48108 USA

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