Detect and quantitatively measure the amount of cAMP present in cell based assay systems
Features
  • A time-resolved fluorescence energy transfer assay for the detection of cAMP in cell based assay systems
  • Reagents supplied to run up to 1,000 assays in 384 well plates OR 2,000 assays in 1536 well plates
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Cyclic AMP TR-FRET Kit

Item No. 23042

Kit Booklet (PDF)
Technical Information
Synonyms
  • Adenosine 3',5'-cyclic mononucleotide
  • Adenosine 3',5'-cyclic monophosphate
  • cAMP TR-FRET
Emission
665 nm
Excitation
340 nm
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea

    $550

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    This product has been produced by

    Columbia Biosciences
    Bioanalysis & Assay Development Services
    Your Vision. Our Expertise.
    • Bioanalysis and compound screening using Cayman or qualified commercial assay kits
    • Development and qualification of fit-for-purpose assays in a variety of formats and platforms
    • Experienced scientists dedicated to developing the best strategy for your project
    LEARN MORE
    Product Description

    This kit is designed to detect and quantitatively measure the amount of cyclic AMP (cAMP) present in cell-based assay systems. A europium (EU) labeled monoclonal anti-cAMP antibody (donor) is paired with a fluorescently labeled cAMP analog (Acceptor), and in the absence of any endogenous cAMP, will produce a time-resolved energy transfer signal at 665 nm when excited at 340 nm. If cAMP accumulates in the well due to the activation of cellular adenylyl cyclase, this cAMP will compete with the acceptor and will inhibit the formation of the labeled Donor/Acceptor complex thereby decreasing the amount of the energy transfer signal produced. The overall drop in signal is inversely proportional to the amount of cAMP accumulated.

    WARNING This product is not for human or veterinary use.