A muscarinic receptor agonist
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Acetyl-β-methylcholine (chloride)

Item No. 23092

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-(acetyloxy)-N,N,N-trimethyl-1-propanaminium, monochloride
CAS Number
62-51-1
Synonyms
  • Methacholine
Molecular Formula
C8H18NO2 • Cl
Formula Weight
195.7
Purity
≥95%
Formulation
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: 0.1 mg/mlPBS (pH 7.2): 5 mg/ml
λmax
210 nm
SMILES
CC(OC(C[N+](C)(C)C)C)=O.[Cl-]
InChi Code
InChI=1S/C8H18NO2.ClH/c1-7(11-8(2)10)6-9(3,4)5;/h7H,6H2,1-5H3;1H/q+1;/p-1
InChi Key
JHPHVAVFUYTVCL-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 g

    $41

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    10 g

    $65

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    25 g

    $119

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    Product Description

    Acetyl-β-methylcholine is a muscarinic (M) receptor agonist.1 In wild-type mice, acetyl-β-methylcholine decreases heart rate and blood pressure and increases the airway pressure time index, a measure of bronchoconstriction, in a dose-dependent manner. Cardiac and pulmonary responses are abolished in M2 and M3 knockout mice, respectively, suggesting that acetyl-β-methylcholine acts on cardiac M2 and pulmonary M3 receptors. Formulations containing acetyl-β-methylcholine have been used to diagnose asthma and to monitor response to asthma therapies.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Fisher, J.T., Vincent, S.G., Gomeza, J., et al. Loss of vagally mediated bradycardia and bronchoconstriction in mice lacking M2 or M3 muscarinic acetylcholine receptors. FASEB J. 18(67), 711-713 (2004).

    2. Choi, S.H., Sheen, Y.H., Kim, M.A., et al. Clinical implications of oscillatory lung function during methacholine bronchoprovocation testing of preschool children. Biomed. Res. Int. 2017, 1-9 (2017).