A fluorogenic substrate for α- and β-galactopyranosaminidase
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4-Methylumbelliferyl 2-Acetamido-2-deoxy-α-D-galactopyranoside

Item No. 23099

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-[[2-(acetylamino)-2-deoxy-α-D-galactopyranosyl]oxy]-4-methyl-2H-1-benzopyran-2-one
CAS Number
124223-99-0
Synonyms
  • 4-MU-2-Acetoamido-2-deoxy-α-Gal
Molecular Formula
C18H21NO8
Formula Weight
379.4
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 1 mg/mlPBS (pH 7.2): 0.1 mg/ml
λmax
203, 318 nm
SMILES
O=C1C=C(C)C2=CC=C(O[C@@H]3[C@H](NC(C)=O)[C@@H](O)[C@@H](O)[C@@H](CO)O3)C=C2O1
InChi Code
InChI=1S/C18H21NO8/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21)/t13-,15-,16+,17-,18+/m1/s1
InChi Key
QCTHLCFVVACBSA-LHKMKVQPSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $32

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    5 mg

    $152

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    10 mg

    $288

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    25 mg

    $680

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    Product Description

    4-Methylumbelliferyl 2-acetoamido-2-deoxy-α-D-galactopyranoside (4-MU-2-acetoamido-2-deoxy-α-Gal) is a fluorogenic substrate used to quantify α- and β-galactopyranosaminidase activity.1 4-MU-2-acetoamido-2-deoxy-α-Gal is cleaved by α- and β-galactopyranosaminidase to release the fluorescent moiety 4-MU. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Szweda, R., Spohr, U., Lemieux, R.U., et al. Synthesis of 4-methylumbelliferyl glycosides for the detection of α- and β-D-galactopyranosaminidases. Can. J. Chem. 67(9), 1388-1391 (1989).

    2. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule. J. Spectrosc. 147128 (2013).