A fluorescent probe
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Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes

Research Area

Application

Fluorescence

Rhodamine B hydrazide

Item No. 23133

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-amino-3',6'-bis(diethylamino)-spiro[1H-isoindole-1,9'-[9H]xanthen]-3(2H)-one
CAS Number
74317-53-6
Molecular Formula
C28H32N4O2
Formula Weight
456.6
Purity
≥98%
Emission
578 nm
Excitation
510 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMF:PBS(pH7.2) (1:2): 0.33 mg/mlDMSO: 20 mg/mlEthanol: 0.1 mg/ml
λmax
239, 274, 314 nm
SMILES
CCN(CC)C1=CC2=C(C=C1)C3(C(C=CC=C4)=C4C(N3N)=O)C5=CC=C(N(CC)CC)C=C5O2
InChi Code
InChI=1S/C28H32N4O2/c1-5-30(6-2)19-13-15-23-25(17-19)34-26-18-20(31(7-3)8-4)14-16-24(26)28(23)22-12-10-9-11-21(22)27(33)32(28)29/h9-18H,5-8,29H2,1-4H3
InChi Key
WTDHTIVYKKLOTC-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    5 mg

    $36

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    10 mg

    $63

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    25 mg

    $110

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    100 mg

    $336

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    Product Description

    Rhodamine B hydrazide is a water-soluble fluorescent probe with excitation/emission spectra of 510/578 nm, respectively.1 It has been used for the detection of copper, peroxynitrite, nitric oxide, hydrogen peroxide, glucose, diacetyl, and hemoglobin.1,2,3,4,5,6

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dujols, V., Ford, F., and Czarnik, A.W. A long-wavelength fluorescent chemodosimeter aelective for Cu(II) ion in water. J. Am. Chem. Soc. 119(31), 7386-7387 (1997).

    2. Yang, X.-F., Guo, X.-Q., and Zaho, Y.-B. Development of a novel rhodamine-type fluorescent probe to determine peroxynitrite. Talanta 57(5), 883-890 (2002).

    3. Wu, C.M., Chen, Y.H., Dayananda, K., et al. Sensitivity evaluation of rhodamine B hydrazide towards nitric oxide and its application for macrophage cells imaging. Anal. Chim. Acta 708(1-2), 141-148 (2011).

    4. Chen, X., and Jianli, Z. Application of rhodamine B hydrazide as a new fluorogenic indicator in the highly sensitive determination of hydrogen peroxide and glucose based on the catalytic effect of iron(III)-tetrasulfonato-phthalocyanine. Microchim. Acta. 157, 133-138 (2007).

    5. Li, X., Duerkop, A., and Wolfbeis, O.S. A fluorescent probe for diacetyl detection. J. Fluoresc. 19(4), 601-606 (2009).

    6. Yang, X.-F., Guo, X.-Q., and Li, H. Fluorimetric determination of hemoglobin using spiro form rhodamine B hydrazide in a micellar medium. Talanta 61(4), 439-445 (2003).

    Product Citations

    Georgiou, C.D., Zisimopoulos, D., Argyropoulou, V., et al. Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay. Redox Biol. 17, 236-245 (2018).