An enzyme substrate
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4-Nitrophenyl β-D-Cellobioside

Item No. 23378

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-nitrophenyl 4-O-β-D-glucopyranosyl-β-D-glucopyranoside
CAS Number
3482-57-3
Synonyms
  • p-Nitrophenyl β-D-Cellobioside
  • para-Nitrophenyl β-D-Cellobioside
Molecular Formula
C18H25NO13
Formula Weight
463.4
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
PBS (pH 7.2): 1 mg/ml
λmax
220, 302 nm
SMILES
OC[C@H]1O[C@@H](OC2=CC=C([N+]([O-])=O)C=C2)[C@H](O)[C@@H](O)[C@@H]1O[C@]3([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O
InChi Code
InChI=1S/C18H25NO13/c20-5-9-11(22)12(23)14(25)18(30-9)32-16-10(6-21)31-17(15(26)13(16)24)29-8-3-1-7(2-4-8)19(27)28/h1-4,9-18,20-26H,5-6H2/t9-,10-,11-,12+,13-,14-,15-,16-,17-,18+/m1/s1
InChi Key
IAYJZWFYUSNIPN-KFRZSCGFSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    4-Nitrophenyl β-D-cellobioside is a disaccharide and an enzyme substrate.1 It has been used to characterize the function of endoglucanase 1 from T. harzianum, β-glucosidase from T. xylanolyticum, and glycoside hydrolase family 3 (GH3) aryl β-glucosidases from A. oryzae.1,2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Charoenwattanasatien, R., Penthaisong, S., Breen, I., et al. Bacterial β-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2). ACS Chem. Biol. 11(7), 1891-1900 (2016).

    2. Kudo, K., Watanabe, A., Ujiie, S., et al. Purification and enzymatic characterization of secretory glycoside hydrolase family 3 (GH3) aryl β-glucosidases screened from Aspergillus oryzae genom. J. Biosci. Bioeng. 120(6), 614-623 (2015).

    3. Pellegrini, V.O., Serpa, V.I., Godoy, A.S., et al. Recombinant Trichoderma harzianum endoglucanase I (Cel7B) is a highly acidic and promiscuous carbohydrate-active enzyme. Appl. Microbiol. Biotechnol. 99(22), 9591-9604 (2015).