A SHAPE reagent
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FAI

Item No. 23429

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
1H-imidazol-1-yl(2-methyl-3-furanyl)-methanone
CAS Number
1415238-77-5
Synonyms
  • Furoyl Acylimidazole
  • 5S rRNA Modificator
Molecular Formula
C9H8N2O2
Formula Weight
176.2
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlEthanol: 30 mg/mlEthanol:PBS (pH7.2) (1:7): 0.12 mg/ml
λmax
268 nm
SMILES
O=C(N1C=CN=C1)C2=C(C)OC=C2
InChi Code
InChI=1S/C9H8N2O2/c1-7-8(2-5-13-7)9(12)11-4-3-10-6-11/h2-6H,1H3
InChi Key
OOBPIWAAJBRELM-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $36

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    5 mg

    $153

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    10 mg

    $268

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    25 mg

    $587

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    Product Description

    FAI is a selective hydroxyl acylation analyzed by primer extension (SHAPE) electrophile reagent used to map RNA structures in vivo by forming stable 2’-hydroxy adducts with RNA, blocking reverse transcriptase elongation.1,2 Its activity can be quenched by dithiothreitol (DTT) in vitro and in living cells.2 FAI has been used to probe the secondary structure of mouse embryonic stem cell 5S rRNA in vitro.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Spitale, R.C., Crisalli, P., Flynn, R.A., et al. RNA SHAPE analysis in living cells. Nat. Chem. Biol. 9(1), 18-20 (2013).

    2. Chan, D., Feng, C., Zhen, Y., et al. Comparative analysis reveals furoyl in vivo selective hydroxyl acylation analyzed by primer extension reagents form stable ribosyl ester adducts. Biochemistry 56(13), 1811-1814 (2017).