For immunochemical detection of cGAS
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cGAS Monoclonal Antibody (Clone 5G10)

Item No. 23853

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • C6orf150
  • cGAMP synthase
  • h-cGAS
  • Cyclic GMP-AMP synthase
  • Mab-21 domain-containing protein 1
  • MB21D1
Immunogen
Full length human recombinant protein
Clone Designation
5G10
Amino Acids
2-543 (full length)
Formulation (Request formulation change)
300 µg of protein G-purified IgG
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Mouse
Isotype
IgG2A
Applications
IF, IP, and WB
Species Reactivity
(+) Human cGAS
UniProt Accession №
Q8N884
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    300 µg

    $555

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    Cayman Chemical
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    Product Description

    Cyclic GMP-AMP (cGAMP) synthase (cGAS; Item No. 22810) is a nucleotidyltransferase located in the cytosol that acts as a cytosolic DNA sensor to detect foreign DNA from microbial pathogens as part of the innate immune response.1,2 Upon binding to cytosolic DNA, cGAS produces the cyclic dinucleotide second messenger cGAMP, which activates stimulator of interferon genes (STING), leading to activation of the type I interferon (IFN) pathway.1,2,3 In vitro, fibroblasts, macrophages, and dendritic cells isolated from cGAS knockout (cGAS-/-) mice do not produce type I IFNs following DNA transfection or DNA virus infection.4 Similarly, cells containing a frame-shift mutation in the cGAS locus fail to mount an immune response to HIV and other retroviruses.5 In vivo, cGAS-/- mice infected with herpes simplex virus 1 (HSV-1) have lower levels of IFN-α and IFN-β, shorter survival times, and higher post-mortem levels of HSV-1 in the brain.4 Cayman’s cGAS Monoclonal Antibody (Clone 5G10) recognizes the full length human cGAS protein at ~59 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sun, L., Wu, J., Du, F., et al. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science 339(6121), 786-791 (2013).

    2. Wu, J., Sun, L., Chen, X., et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science 339(6121), 826-830 (2013).

    3. Ablasser, A., Goldeck, M., Cavlar, T., et al. cGAS produces a 2'-5'-linked cyclic dinucleotide second messenger that activates STING. Nature 498(7454), 380-384 (2013).

    4. Li, X.-D., Wu, J., Gao, D., et al. Pivotal roles of cGAS-cGAMP signaling in antiviral defense and immune adjuvant effects. Science 341(6152), 1390-1394 (2013).

    5. Gao, D., Wu, J., Wu, Y.-T., et al. Cyclic GMP-AMP synthase is an innate immune sensor of HIV and other retroviruses. Science 341(6148), 903-906 (2013).