Active, pure human recombinant enzyme
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PAF Acetylhydrolase 2 (human, recombinant)

Item No. 24292

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • PAF-AH2
  • HSD-PLA2
  • Platelet-Activating Factor Acetylhydrolase 2, Cytoplasmic
  • Serine-Dependent Phospholipase A2
Purity
≥80% estimated by SDS page
Source
Active recombinant N-terminal His-tagged protein expressed in E. coli
Amino Acids
2-392
MW
46.1 kDa
Storage Buffer
50 mM HEPES, pH 8.0, with 300 mM sodium chloride and 20% glycerol
UniProt Accession №
Q99487
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $528

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    Product Description

    PAF acetylhydrolase 2 (PAF-AH2) is a serine-dependent, intracellular-type platelet-activating factor (PAF) acetylhydrolase that is expressed in a variety of tissue and cell types but is most abundant in the liver, kidney, testis, and intestines.1,2,3,4 It contains an N-terminal myristoylation site and can translocate from the cytosol to cellular membranes in response to various stressors including UVB radiation, LPS, or oxidative stress, during which PAF-AH2 localizes to the endoplasmic reticulum and Golgi apparatus.2,5 PAF-AH2 hydrolyzes and inactivates PAF, a phospholipid mediator involved in a variety of biological activities, including inflammation, smooth muscle contraction, and fetal development.2,3,6 It also hydrolyzes phospholipids with short-chain or oxidized fatty acids at the sn-2 position.1,2 PAF-AH2 has demonstrated protective activities in various in vitro and in vivo models of oxidative stress.2,4,5 Overexpression of PAF-AH2 reduces cytotoxicity and apoptosis induced by tert-butylhydroperoxide (t-BuOOH) in CHO-K1 cells.5 Conversely, PAF-AH2-deficient (Pafah2-/-) mouse embryonic fibroblasts (MEFs) are more susceptible to t-BuOOH-induced cell death than wild-type MEFs.4 In addition, Pafah2-/- mice exhibit increased hepatic necrosis compared to wild-type animals in a mouse model of carbon tetrachloride-induced hepatic injury.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Rice, S.Q., Southan, C., Boyd, H.F., et al. Expression, purification and characterization of a human serine-dependent phospholipase A2 with high specificity for oxidized phospholipids and platelet activating factor. Biochem. J. 330(Pt 3), 1309-1315 (1998).

    2. Kono, N., and Arai, H. Intracellular platelet-activating factor acetylhydrolase, type II: A unique cellular phospholipase A2 that hydrolyzes oxidatively modified phospholipids. Enzymes 38, 43-54 (2015).

    3. McIntyre, T.M., Prescott, S.M., and Stafforini, D.M. The emerging roles of PAF acetylhydrolase. J. Lipid Res. 50(Suppl), S255-S259 (2009).

    4. Kono, N., Inoue, T., Yoshida, Y., et al. Protection against oxidative stress-induced hepatic injury by intracellular type II platelet-activating factor acetylhydrolase by metabolism of oxidized phospholipids in vivo. The Journal of Biological Chemisty 283(3), 1628-1636 (2008).

    5. Matsuzawa, A., Hattori, K., Aoki, J., et al. Protection against oxidative stress-induced cell death by intracellular platelet-activating factor-acetylhydrolase II. The Journal of Biological Chemisty 272(51), 32315-32320 (1997).

    6. Hattori, K., Adachi, H., Matsuzawa, A., et al. cDNA cloning and expression of intracellular platelet-activating factor (PAF) acetylhydrolase II. Its homology with plasma PAF acetylhydrolase. The Journal of Biological Chemisty 271(51), 33032-33038 (1996).