A caspase-9 substrate
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Ac-LEHD-pNA (trifluoroacetate salt)

Item No. 24570

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-acetyl-L-leucyl-L-α-glutamyl-L-histidyl-N-(4-nitrophenyl)-L-α-asparagine, 2,2,2-trifluoroacetate
Synonyms
  • Ac-Leu-Glu-His-Asp-pNA
  • Caspase-9 Chromogenic Substrate I
Molecular Formula
C29H38N8O11 • XCF3COOH
Formula Weight
674.7
Purity
≥95%
Formulation (Request formulation change)
A lyophilized powder
Solubility(Learn about Variance in Solubility)
Water: 1 mg/ml
SMILES
CC(C)C[C@@H](C(N[C@H](C(N[C@@H](CC1=CN=CN1)C(N[C@@H](CC(O)=O)C(NC2=CC=C([N+]([O-])=O)C=C2)=O)=O)=O)CCC(O)=O)=O)NC(C)=O.OC(C(F)(F)F)=O
InChi Code
InChI=1S/C29H38N8O11.C2HF3O2/c1-15(2)10-21(32-16(3)38)28(45)34-20(8-9-24(39)40)26(43)35-22(11-18-13-30-14-31-18)29(46)36-23(12-25(41)42)27(44)33-17-4-6-19(7-5-17)37(47)48;3-2(4,5)1(6)7/h4-7,13-15,20-23H,8-12H2,1-3H3,(H,30,31)(H,32,38)(H,33,44)(H,34,45)(H,35,43)(H,36,46)(H,39,40)(H,41,42);(H,6,7)/t20-,21-,22-,23-;/m0./s1
InChi Key
GNOHPGPOHKXXOO-JSEXCYGQSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $49

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    Product Description

    Ac-LEHD-pNA is a substrate for caspase-9.1 Caspase-9 preferentially binds to and cleaves the Leu-Glu-His-Asp (LEHD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity.1,2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hsu, Y.L., Kuo, P.L., Lin, L.T., et al. Asiatic acid, a triterpene, induces apoptosis and cell cycle arrest through activation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways in human breast cancer cells. J. Pharmacol. Exp. Ther. 313(1), 333-344 (2005).

    2. Poręba, M., Stróżyk, A., Salvesen, G.S., et al. Caspase substrates and inhibitors. Cold Spring Harb. Perspect. Biol. 5(8), A008680 (2013).

    3. Yu, M.-S., Suen, K.-C., Kwok, N.-S., et al. Beta-amyloid peptides induces neuronal apoptosis via a mechanism independent of unfolded protein responses. Apoptosis 11(5), 687-700 (2006).