An antimicrobial peptide
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Defensin HNP-1 (human) (trifluoroacetate salt)

Item No. 24572

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
L-alanyl-L-cysteinyl-L-tyrosyl-L-cysteinyl-L-arginyl-L-isoleucyl-L-prolyl-L-alanyl-L-cysteinyl-L-isoleucyl-L-alanylglycyl-L-α-glutamyl-L-arginyl-L-arginyl-L-tyrosylglycyl-L-threonyl-L-cysteinyl-L-isoleucyl-L-tyrosyl-L-glutaminylglycyl-L-arginyl-L-leucyl-L-tryptophyl-L-alanyl-L-phenylalanyl-L-cysteinyl-L-cysteine, cyclic (2→30),(4→19),(9→29)-tris(disulfide), trifluoroacetate salt
Synonyms
  • DEFA1 Protein
  • α-Defensin 1
  • Human Neutrophil Peptide 1
  • Neutrophil Defensin 1
Molecular Formula
C150H222N44O38S6 • XCF3COOH
Formula Weight
3442.0
Purity
≥95%
Formulation (Request formulation change)
A lyophilized powder
Solubility(Learn about Variance in Solubility)
Water: 1 mg/ml
SMILES
[H]N[C@H](C(N[C@H](C(N[C@H](C(N[C@H](C(N[C@@H](CCCNC(N)=N)C(N[C@]([C@H](CC)C)([H])C(N1CCC[C@H]1C(N[C@H](C(N[C@H](C(N[C@]([C@H](CC)C)([H])C(N[C@H](C(NCC(N[C@@H](CCC(O)=O)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CCCNC(N)=N)C(N[C@H](C(NCC(N[C@]([C@@H](C)O)([H])C2=O)=O)=O)CC3=CC=C(O)C=C3)=O)=O)=O)=O)=O)C)=O)=O)CSSC[C@H](N4)C5=O)=O)C)=O)=O)=O)=O)CSSC[C@H](N2)C(N[C@]([C@H](CC)C)([H])C(N[C@H](C(N[C@@H](CCC(N)=O)C(NCC(N[C@@H](CCCNC(N)=N)C(N[C@@H](CC(C)C)C(N[C@H](C(N[C@H](C(N[C@H](C4=O)CC6=CC=CC=C6)=O)C)=O)CC7=CNC8=C7C=CC=C8)=O)=O)=O)=O)=O)CC9=CC=C(O)C=C9)=O)=O)=O)CC%10=CC=C(O)C=C%10)=O)CSSC[C@H](N5)C(O)=O)=O)C.OC(C(F)(F)F)=O
InChi Code
InChI=1S/C150H222N44O38S6.C2HF3O2/c1-14-74(6)116-142(227)171-78(10)121(206)166-64-112(200)173-97(49-51-115(203)204)129(214)175-93(32-23-53-162-148(155)156)127(212)174-94(33-24-54-163-149(157)158)128(213)180-99(59-83-36-42-87(196)43-37-83)125(210)168-66-114(202)190-119(81(13)195)144(229)188-108-71-236-233-68-105-136(221)176-95(34-25-55-164-150(159)160)130(215)193-118(76(8)16-3)145(230)194-56-26-35-110(194)141(226)170-80(12)123(208)185-107(139(224)191-116)70-235-234-69-106(138(223)189-109(146(231)232)72-238-237-67-104(184-120(205)77(9)151)137(222)181-101(135(220)186-105)60-84-38-44-88(197)45-39-84)187-134(219)100(58-82-27-18-17-19-28-82)178-122(207)79(11)169-131(216)103(62-86-63-165-91-30-21-20-29-90(86)91)182-132(217)98(57-73(4)5)179-126(211)92(31-22-52-161-147(153)154)172-113(201)65-167-124(209)96(48-50-111(152)199)177-133(218)102(61-85-40-46-89(198)47-41-85)183-143(228)117(75(7)15-2)192-140(108)225;3-2(4,5)1(6)7/h17-21,27-30,36-47,63,73-81,92-110,116-119,165,195-198H,14-16,22-26,31-35,48-62,64-72,151H2,1-13H3,(H2,152,199)(H,166,206)(H,167,209)(H,168,210)(H,169,216)(H,170,226)(H,171,227)(H,172,201)(H,173,200)(H,174,212)(H,175,214)(H,176,221)(H,177,218)(H,178,207)(H,179,211)(H,180,213)(H,181,222)(H,182,217)(H,183,228)(H,184,205)(H,185,208)(H,186,220)(H,187,219)(H,188,229)(H,189,223)(H,190,202)(H,191,224)(H,192,225)(H,193,215)(H,203,204)(H,231,232)(H4,153,154,161)(H4,155,156,162)(H4,157,158,163)(H4,159,160,164);(H,6,7)/t74-,75-,76-,77-,78-,79-,80-,81+,92-,93-,94-,95-,96-,97-,98-,99-,100-,101-,102-,103-,104-,105-,106-,107-,108-,109-,110-,116-,117-,118-,119-;/m0./s1
InChi Key
BRFFERRJYQGMQB-GCQWOBDHSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    Defensin HNP-1 is a peptide secreted by polymorphonuclear leukocytes (PMNs) that has antimicrobial properties.1 It induces lysis of mammalian cells when used at concentrations of 25 and 100 μg/ml, respectively.2 It also completely inhibits secretion of the exotoxin superantigen TSS toxin-1 (TSST-1) from S. aureus when used at a concentration of 50 ng/ml and inhibits anthrax lethal toxin (IC50 = 148 nM).3,4 HNP-1 binds to recombinant HIV-1 envelope glycoprotein (gp120) and human CD4 (Kds = 23.2 and 12.9 nM, respectively).5 It induces lysis and leakage from liposomes composed of dipalmitoyl phosphatidylcholine (DPPC; Item No. 10009473) and dioleyl phosphatidylserine (DOPS).6 In vivo, HNP-1 (5 μg) increases interferon α (IFN-α) production by human plasmacytoid dendritic cells (pDCs) injected into nude mice.7

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lehrer, R.I. Primate defensins. Nat. Rev. Microbiol. 2(9), 727-738 (2004).

    2. Lichtenstein, A., Ganz, T., Selsted, M.E., et al. In vitro tumor cell cytolysis mediated by peptide defensins of human and rabbit granulocytes. Blood 68(6), 1407-1410 (1986).

    3. Merriman, J.A., Nemeth, K.A., and Schlievert, P.M. Novel antimicrobial peptides that inhibit gram positive bacterial exotoxin synthesis. PLoS One 9(4), (2014).

    4. Wei, G., Pazgier, M., De Leeuw, E., et al. Trp-26 imparts functional versatility to human α-defensin HNP1. The Journal of Biological Chemisty 285(21), 16275-16285 (2010).

    5. Wang, W., Owen, S.M., Rudolph, D.L., et al. Activity of ɑ- and θ-defensins against primary isolates of HIV-1. J. Immunol. 173(1), 515-520 (2004).

    6. Fujii, G., Selsted, M.E., and Eisenberg, D. Defensins promote fusion and lysis of negatively charged membranes. Protein Sci. 2(8), 1301-1312 (1993).

    7. Wang, F., Qiao, L., Lv, X., et al. Alarmin human α defensin HNP1 activates plasmacytoid dendritic cells by triggering NF-κB and IRF1 signaling pathways. Cytokine 83, 53-60 (2016).