A pure human recombinant protein
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cGAS (161-522) (human recombinant)

Item No. 25001

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • C6orf150
  • 2’3’-cGAMP Synthase
  • cGAMP Synthase
  • h-cGAS
  • Cyclic GMP-AMP Synthase
  • Mab-21 domain-containing protein 1
  • MB21D1
Purity
≥90% estimated by SDS-PAGE
Source
N-terminal histidine-tagged human cGAS protein (truncated), purified from E. coli
Amino Acids
161-522 (truncated)
MW
44.6 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 8.0, 150 mM sodium chloride, 1 mM DTT, and 10% glycerol
UniProt Accession №
Q8N884
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $469

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    Product Description

    Cyclic GMP-AMP (cGAMP) synthase (cGAS) (161-522) is a truncated form of cGAS (Item No. 22810) that contains the nucleotidyltransferase and Mab21 domains.1 cGAS is a nucleotidyltransferase located in the cytosol that acts as a cytosolic DNA sensor to detect foreign DNA from microbial pathogens as part of the innate immune response.2,3 Upon binding to cytosolic DNA, cGAS produces the cyclic dinucleotide second messenger cGAMP, which activates stimulator of interferon genes (STING), leading to activation of the type I interferon (IFN) pathway.2,3,4 In vitro, fibroblasts, macrophages, and dendritic cells isolated from cGAS knockout (cGAS-/-) mice do not produce type I IFNs following DNA transfection or DNA virus infection.5 Similarly, cells containing a frame-shift mutation in the cGAS locus fail to mount an immune response to HIV and other retroviruses.6 In vivo, cGAS-/- mice infected with herpes simplex virus 1 (HSV-1) have lower levels of IFN-α and IFN-β, shorter survival times, and higher post-mortem levels of HSV-1 in the brain.5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Tao, J., Zhang, X.-W., Jin, J., et al. Nonspecific DNA Binding of cGAS N Terminus Promotes cGAS Activation. J. Immunol. 198(9), 3627-3636 (2017).

    2. Sun, L., Wu, J., Du, F., et al. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science 339(6121), 786-791 (2013).

    3. Wu, J., Sun, L., Chen, X., et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science 339(6121), 826-830 (2013).

    4. Ablasser, A., Goldeck, M., Cavlar, T., et al. cGAS produces a 2'-5'-linked cyclic dinucleotide second messenger that activates STING. Nature 498(7454), 380-384 (2013).

    5. Li, X.-D., Wu, J., Gao, D., et al. Pivotal roles of cGAS-cGAMP signaling in antiviral defense and immune adjuvant effects. Science 341(6152), 1390-1394 (2013).

    6. Gao, D., Wu, J., Wu, Y.-T., et al. Cyclic GMP-AMP synthase is an innate immune sensor of HIV and other retroviruses. Science 341(6148), 903-906 (2013).