A fluorescent DNA probe for live and fixed cells
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Nuclear Yellow

Item No. 25178

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]-benzenesulfonamide, trihydrochloride
CAS Number
74681-68-8
Synonyms
  • Hoechst S769121
Molecular Formula
C25H25N7O2S • 3HCl
Formula Weight
597.0
Emission
495 nm
Excitation
355 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: SolubleWater: Soluble
SMILES
CN1CCN(C2=CC(N=C(C3=CC(N=C(C4=CC=C(S(N)(=O)=O)C=C4)N5)=C5C=C3)N6)=C6C=C2)CC1.Cl.Cl.Cl
InChi Code
InChI=1S/C25H25N7O2S.3ClH/c1-31-10-12-32(13-11-31)18-5-9-21-23(15-18)30-25(28-21)17-4-8-20-22(14-17)29-24(27-20)16-2-6-19(7-3-16)35(26,33)34;;;/h2-9,14-15H,10-13H2,1H3,(H,27,29)(H,28,30)(H2,26,33,34);3*1H
InChi Key
NZVGXJAQIQJIOY-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $81

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    10 mg

    $152

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    25 mg

    $337

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    Product Description

    Nuclear yellow is a DNA-selective dye that is used to label DNA content in live and fixed cells. Upon binding to DNA, nuclear yellow fluoresces, and this fluorescence can be measured using fluorescence microscopy, microplate fluorometry, or flow cytometry. Nuclear yellow has commonly been used in combination with retrograde tracers for two-color neuronal mapping.1 It can also be used to photoconvert diaminobenzidine (DAB) into an electron-dense reaction product for light and electron microscopy applications.2 Nuclear yellow displays excitation/emission maxima of 355/495 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Pollin, B., Laplante, S., Cesaro, P., et al. Simultaneous visualization of Nuclear yellow and iron-dextran complex for demonstration of branched neurons by retrograde axonal transport. J. Neurosci. Methods 8(3), 205-209 (1983).

    2. Lübke, J. Photoconversion of diaminobenzidine with different fluorescent neuronal markers into a light and electron microscopic dense reaction product. Microsc. Res. Tech. 24(1), 2-14 (1993).