A colorimetric substrate for lipases
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4-Nitrophenyl Butyrate

Item No. 25439

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
butanoic acid, 4-nitrophenyl ester
CAS Number
2635-84-9
Synonyms
  • p-Nitrophenyl Butyrate
  • para-Nitrophenyl Butyrate
  • 4-NPB
  • NSC 6867
Molecular Formula
C10H11NO4
Formula Weight
209.2
Purity
≥95%
Formulation (Request formulation change)
A neat oil
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlDMSO:PBS (pH 7.2) (1:1): 0.5 mg/mlEthanol: 15 mg/ml
λmax
270 nm
SMILES
O=C(CCC)OC1=CC=C([N+]([O-])=O)C=C1
InChi Code
InChI=1S/C10H11NO4/c1-2-3-10(12)15-9-6-4-8(5-7-9)11(13)14/h4-7H,2-3H2,1H3
InChi Key
DVDUMIQZEUTAGK-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

    $54

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    5 g

    $223

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    10 g

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    25 g

    $913

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    Product Description

    4-Nitrophenyl butyrate (4-NPB) is a colorimetric substrate for lipases.1,2 Upon hydrolysis, 4-NPB is converted into p-nitrophenoxide, which can be monitored using UV-Vis spectrophotometry at 400 nm to quantify lipase activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sterri, S.H., Johnsen, B.A., and Fonnum, F. A radiochemical assay method for carboxylesterase, and comparison of enzyme activity towards the substrates methyl [1-14C] butyrate and 4-nitrophenyl butyrate. Biochem. Pharmacol. 34(15), 2779-2785 (1985).

    2. Das, A., and Chakrabarti, K. A cold tolerant lipase develops enhanced activity, thermal tolerance and solvent stability in the presence of calcium nanoparticles: Alternative approach to genetic modulation. Biocatal. Agric. Biotechnol. 15, (2018).