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Lucifer yellow CH is a fluorescent probe that contains a free hydrazido group that reacts with aliphatic aldehydes at room temperature.1 Lucifer yellow CH displays excitation/emission maxima of 430/540 nm, respectively. It has been used as a biological tracer to monitor the branching pattern and regeneration of neurons, gap junction detection and characterization, and selective ablation of cells following aldehyde fixation.1,2
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1. Lucifer dyes—highly fluorescent dyes for biological tracing. Nature 292(5818), 17-21 (1981).
2. Lucifer yellow – an angel rather than the devil. J. Cell. Mol. Med. 16(1), 22-31 (2012).