A fluorescent biotin derivative
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Fluorescein Biotin (triethylamine salt)

Item No. 25574

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(3aS,4S,6aR)-N-[6-[[5-[[[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)amino]thioxomethyl]amino]pentyl]amino]-6-oxohexyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide, ditriethylamine salt
Synonyms
  • FITC Biotin
Molecular Formula
C42H50N6O8S2 • 2C6H15N
Formula Weight
1033.4
Purity
≥90%
Emission
535 nm
Excitation
485 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: SolubleDMSO: SolubleMethanol: SolubleWater: Slightly soluble
λmax
222 nm
SMILES
S=C(NCCCCCNC(CCCCCNC(CCCC[C@H]1[C@]2([H])[C@](NC(N2)=O)([H])CS1)=O)=O)NC3=CC=C4C(C(OC45C(C=CC(O)=C6)=C6OC7=C5C=CC(O)=C7)=O)=C3.CCN(CC)CC.CCN(CC)CC
InChi Code
InChI=1S/C42H50N6O8S2.2C6H15N/c49-26-13-16-30-33(22-26)55-34-23-27(50)14-17-31(34)42(30)29-15-12-25(21-28(29)39(53)56-42)46-41(57)45-20-8-2-7-19-44-36(51)10-3-1-6-18-43-37(52)11-5-4-9-35-38-32(24-58-35)47-40(54)48-38;2*1-4-7(5-2)6-3/h12-17,21-23,32,35,38,49-50H,1-11,18-20,24H2,(H,43,52)(H,44,51)(H2,45,46,57)(H2,47,48,54);2*4-6H2,1-3H3/t32-,35-,38-;;/m0../s1
InChi Key
JFQXZJSGJBIBAY-SFBFSKNASA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $156

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    Product Description

    Fluorescein biotin (FITC biotin) is a fluorescent biotin derivative and avidin and streptavidin ligand that contains a FITC fluorophore which displays excitation/emission maxima of 485/535 nm, respectively.1 FITC fluorescence is quenched upon binding to avidin or streptavidin and decreases in fluorescence intensity have been used to quantify biotin binding stoichiometry.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Chu, Y.H., Lees, W.J., Stassinopoulos, A., et al. Using affinity capillary electrophoresis to determine binding stoichiometries of protein-ligand interactions. Biochemistry 33(35), 10616-10621 (1994).

    Product Citations

    Sloas, D.C., Tran, J.C., Marzilli, A.M., et al. Tension-tuned receptors for synthetic mechanotransduction and intercellular force detection. Nat. Biotechnol. 41(9), 1287-1295 (2023).