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RIG-I helicase domain (human, recombinant)

Item No. 25620

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • DEAD Box Protein 58
  • Probable ATP-dependent RNA Helicase DDX58
  • Retinoic Acid-inducible Gene 1 Protein
Purity
≥80% estimated by SDS-PAGE
Source
Recombinant N-terminal histidine-tagged RIG-I helicase domain purified from E. coli
Amino Acids
232-794
MW
66.47 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 8.0, with 150 mM sodium chloride and 10% glycerol
UniProt Accession №
O95786
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $522

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    Product Description

    Retinoic acid-inducible gene I (RIG-I), also known as DDX58, is a cytosolic DExD/H-box RNA helicase and an immune sensing receptor encoded by DDX58 in humans.1 It is composed of a C-terminal repressor domain, a central RNA helicase domain, and two N-terminal caspase recruitment domains (CARDs).1,2 Following recognition of viral dsRNA by the C-terminal and helicase domains, the helicase domain induces an ATP-dependent conformational change, allowing for interaction of the CARD domains with mitochondrial antiviral-signaling protein (MAVS) and induction of a type I interferon (IFN) response.1,2,3 A single amino acid substitution of phenylalanine for cysteine at position 268 in the RIG-I helicase domain (RIG-IC268F) leads to enhanced NF-κB activity and expression of IFN-β in basal and poly (I:C)-stimulated cells.4 Overexpression of RIG-IC268F in human trabecular meshwork (HTM) cells induces cell death and is positively correlated with glaucoma, a core symptom in the type I interferonopathy atypical Singleton-Merten syndrome.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hartmann, G. Nucleic acid immunity. Advances in Immunology 133, 121-169 (2017).

    2. Matsumiya, T., and Stafforini, D.M. Function and regulation of retinoic acid-inducible gene-I. Crit. Rev. Immunol. 30(6), 489-513 (2010).

    3. Takeuchi, O., and Akira, S. Innate immunity to virus infection. Immunol. Rev. 277(1), 75-86 (2009).

    4. Lu, C., and MacDougall, M. RIG-I-like receptor signaling in Singleton-Merten syndrome. Front. Genet. 8:118, (2017).