A fluorescent probe for lipid droplets
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BODIPY 493/503

Item No. 25892

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(T-4)-[2-[1-(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)ethyl]-3,5-dimethyl-1H-pyrrolato-κN]difluoro-boron
CAS Number
121207-31-6
Synonyms
  • ​Pyrromethane 546
Molecular Formula
C14H17BF2N2
Formula Weight
262.1
Purity
≥98%
Emission
503 nm
Excitation
493 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Chloroform: SolubleDMF: SolubleDMSO: SolubleMethanol: Soluble
SMILES
[F][B-]([N]1C2=C(C)C=C1C)([N+](C(C(C)=C3)=C2C)=C3C)[F]
InChi Code
InChI=1S/C14H17BF2N2/c1-8-6-10(3)18-13(8)12(5)14-9(2)7-11(4)19(14)15(18,16)17/h6-7H,1-5H3
InChi Key
DRJHPEGNOPSARR-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $38

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    10 mg

    $60

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    Product Description

    BODIPY 493/503 is a lipophilic fluorescent probe that localizes to polar lipids and can be used to label cellular neutral lipid contents, particularly those localized to lipid droplets, in live and fixed cells.1,2 BODIPY 493/503 is compatible with epifluorescent, confocal, and two-photon microscopy, as well as flow cytometry. It displays excitation/emission maxima of 493/503 nm, respectively, and can be used for live and fixed cell applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Listenberger, L.L., and Brown, D.G. Fluorescent detection of lipid droplets and associated proteins. Curr. Protoc. Cell. Biol. 35(1), 24.22.21-24.22.11 (2007).

    2. Qiu, B., and Simon, M.C. BODIPY 493/503 staining of neutral lipid droplets for microscopy and quantification by flow cytometry. Bio. Protoc. 6(17), (2016).

    Product Citations

    Galigniana, N.M., Abdelhalim, M., Collas, P., et al. Transcriptional and metabolic changes following repeated fasting and refeeding of adipose stem cells highlight adipose tissue resilience. Nutrients 16(24), 4310 (2024).

    Li, H., Dixon, E.E., Wu, H., et al. Comprehensive single-cell transcriptional profiling defines shared and unique epithelial injury responses during kidney fibrosis. Cell Metab. 34(12), 1977-1998 (2022).