A turn-on fluorescent probe to detect hypoxia
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CAY10730

Item No. 26547

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3'-methoxy-6'-[(4-nitrophenyl)methoxy]-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
CAS Number
2206677-06-5
Molecular Formula
C28H19NO7
Formula Weight
481.5
Purity
≥98%
Emission
520 nm
Excitation
454 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 20 mg/mlEthanol: 1 mg/ml
λmax
225, 275 nm
SMILES
COC(C=C1)=CC2=C1C3(C(C=CC=C4)=C4C(O3)=O)C(C=CC(OCC5=CC=C([N+]([O-])=O)C=C5)=C6)=C6O2
InChi Code
InChI=1S/C28H19NO7/c1-33-19-10-12-23-25(14-19)35-26-15-20(34-16-17-6-8-18(9-7-17)29(31)32)11-13-24(26)28(23)22-5-3-2-4-21(22)27(30)36-28/h2-15H,16H2,1H3
InChi Key
RYVPFHHZHVISQN-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $134

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    10 mg

    $240

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    25 mg

    $530

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    50 mg

    $862

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    Cayman Chemical
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    Product Description

    CAY10730 is a turn-on fluorescent probe for the detection of nitroreductase activity, a marker of hypoxia.1 In the presence of nitroreductase, CAY10730 is transformed into a highly fluorescent molecule that displays excitation/emission maxima of 454/520 nm, respectively. It is selective for nitroreductase over a variety of bioanalytes, including glutathione, ascorbic acid, and reactive oxygen species such as H2O2. CAY10730 (1 µM) has been used to detect hypoxia in A549 human lung carcinoma cells.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. More, K.N., Lim, T.-H., Kim, S.-Y., et al. Characteristics of new bioreductive fluorescent probes based on the xanthene fluorophore: Detection of nitroreductase and imaging of hypoxic cells. Dyes Pigm. 151, 245-253 (2018).