A chromogenic substrate for β-galactosidase
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o-Nitrophenyl β-D-Galactopyranoside

Item No. 26624

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-nitrophenyl β-D-galactopyranoside
CAS Number
369-07-3
Synonyms
  • ortho-Nitrophenyl β-D-galactopyranoside
  • o-Nitrophenyl β-D-Galactoside
  • ortho-Nitrophenyl β-D-Galactoside
  • NSC 83631
  • ONPG
Molecular Formula
C12H15NO8
Formula Weight
301.3
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 15 mg/mlEthanol: 0.25 mg/mlPBS (pH 7.2): 2 mg/ml
λmax
212, 256, 310 nm
SMILES
OC[C@H]1O[C@@H](OC2=C([N+]([O-])=O)C=CC=C2)[C@H](O)[C@@H](O)[C@H]1O
InChi Code
InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
InChi Key
KUWPCJHYPSUOFW-YBXAARCKSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

    $36

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    5 g

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    10 g

    $253

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    25 g

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    Product Description

    o-Nitrophenyl β-D-galactopyranoside (ONPG) is a chromogenic substrate for β-galactosidase.1,2 Upon enzymatic cleavage by β-galactosidase, o-nitrophenol is released, which can be quantified by colorimetric detection at 420 nm as a measure of β-galactosidase activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Serebriiskii, I.G., and Golemis, E.A. Uses of lacZ to study gene function: Evaluation of β-galactosidase assays employed in the yeast two-hybrid system. Anal. Biochem. 285(1), 1-15 (2000).

    2. Li, W., Zhao, X., Zou, S., et al. Scanning assay of β-galactosidase activity. Prikl. Biokhim. Mikrobiol. 48(6), 668-672 (2012).