A fluorogenic substrate for the 20S proteasome
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Ac-WLA-AMC

Item No. 26639

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-acetyl-L-tryptophyl-L-leucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-alaninamide
CAS Number
1104011-59-7
Synonyms
  • Acetyl-Trp-Leu-Ala-7-amino-4-Methylcoumarin
  • Ac-Trp-Leu-Ala-AMC
Molecular Formula
C32H37N5O6
Formula Weight
587.7
Purity
≥95%
Emission
430 nm
Excitation
351 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: 10 mM
SMILES
O=C1C=C(C)C2=C(C=C(NC([C@@H](NC([C@H](CC(C)C)NC([C@H](CC3=CNC4=C3C=CC=C4)NC(C)=O)=O)=O)C)=O)C=C2)O1
InChi Code
InChI=1S/C32H37N5O6/c1-17(2)12-26(37-32(42)27(35-20(5)38)14-21-16-33-25-9-7-6-8-24(21)25)31(41)34-19(4)30(40)36-22-10-11-23-18(3)13-29(39)43-28(23)15-22/h6-11,13,15-17,19,26-27,33H,12,14H2,1-5H3,(H,34,41)(H,35,38)(H,36,40)(H,37,42)/t19-,26-,27-/m0/s1
InChi Key
PIJMRHCPKXLMIS-DOYMOWJKSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    500 µg

    $63

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    1 mg

    $118

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    Product Description

    Ac-WLA-AMC is a fluorogenic substrate for the β5c subunit of the 20S proteasome.1 Upon cleavage, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify the activity of the β5c subunit of the 20S proteasome. AMC displays excitation/emission maxima of 351/430 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Blackburn, C., Gigstad, K.M., Hales, P., et al. Characterization of a new series of non-covalent proteasome inhibitors with exquisite potency and selectivity for the 20S β5-subunit. Biochem. J. 430(3), 461-476 (2010).