A fluorogenic substrate for the 20S immunoproteasome
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Ac-ANW-AMC

Item No. 26640

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(S)-N1-((S)-3-(1H-indol-3-yl)-1-((4-methyl-2-oxo-2H-chromen-7-yl)amino)-1-oxopropan-2-yl)-2-((S)-2-acetamidopropanamido)succinamide
Synonyms
  • Ac-Ala-Asn-Trp-AMC
Molecular Formula
C30H32N6O7
Formula Weight
588.6
Purity
≥95%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: 10mM
SMILES
O=C1C=C(C)C2=C(C=C(NC([C@H](CC3=CNC4=C3C=CC=C4)NC([C@H](CC(N)=O)NC([C@H](C)NC(C)=O)=O)=O)=O)C=C2)O1
InChi Code
InChI=1S/C30H32N6O7/c1-15-10-27(39)43-25-12-19(8-9-20(15)25)34-29(41)23(11-18-14-32-22-7-5-4-6-21(18)22)36-30(42)24(13-26(31)38)35-28(40)16(2)33-17(3)37/h4-10,12,14,16,23-24,32H,11,13H2,1-3H3,(H2,31,38)(H,33,37)(H,34,41)(H,35,40)(H,36,42)/t16-,23-,24-/m0/s1
InChi Key
ORTKHFLJSJQDKO-ZCWWJEROSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

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    500 µg

    $63

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    1 mg

    $118

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    Cayman Chemical
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    Product Description

    Ac-ANW-AMC is a fluorogenic substrate for the β5i/LMP7 subunit of the 20S immunoproteasome.1 Upon cleavage, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify the activity of the β5i/LMP7 subunit of the 20S immunoproteasome. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Winter, M.B., La Greca, F., Arastu-Kapur, S., et al. Immunoproteasome functions explained by divergence in cleavage specificity and regulation. eLife 6:e27364, (2017).

    Product Citations

    Rana, P.S., Ignatz-Hoover, J.J., Guo, C., et al. Immunoproteasome activation expands the MHC Class I immunopeptidome, unmasks neoantigens, and enhances T-cell anti-myeloma activity. Mol. Cancer Ther. 23(12), 1743-1760 (2024).