A fluorogenic substrate for isopeptidase T
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Z-LRGG-AMC (trifluoroacetate salt)

Item No. 26641

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[(phenylmethoxy)carbonyl]-L-leucyl-L-arginylglycyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-glycinamide, trifluoroacetate salt
Synonyms
  • Z-Leu-Arg-Gly-Gly-AMC
  • Z-Leu-Arg-Gly-Gly-7-amino-4-methylcoumarin
Molecular Formula
C34H44N8O8 • XCF3COOH
Formula Weight
692.8
Purity
≥95%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMF:PBS(pH 7.2)(1:1): 0.5 mg/mlDMSO: 16 mg/ml
λmax
327 nm
SMILES
O=C1C=C(C)C2=C(C=C(NC(CNC(CNC([C@H](CCCNC(N)=N)NC([C@@H](NC(OCC3=CC=CC=C3)=O)CC(C)C)=O)=O)=O)=O)C=C2)O1.FC(F)(C(O)=O)F
InChi Code
InChI=1S/C34H44N8O8.C2HF3O2/c1-20(2)14-26(42-34(48)49-19-22-8-5-4-6-9-22)32(47)41-25(10-7-13-37-33(35)36)31(46)39-17-28(43)38-18-29(44)40-23-11-12-24-21(3)15-30(45)50-27(24)16-23;3-2(4,5)1(6)7/h4-6,8-9,11-12,15-16,20,25-26H,7,10,13-14,17-19H2,1-3H3,(H,38,43)(H,39,46)(H,40,44)(H,41,47)(H,42,48)(H4,35,36,37);(H,6,7)/t25-,26-;/m0./s1
InChi Key
OHFBLWHOBJRDAB-CCQIZPNASA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $117

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    10 mg

    $196

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    25 mg

    $343

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    50 mg

    $627

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    Product Description

    Z-LRGG-AMC is a fluorogenic substrate for isopeptidase T.1 Upon enzymatic cleavage by isopeptidase T, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify isopeptidase T activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Stein, R.L., Chen, Z., and Melandri, F. Kinetic studies of isopeptidase T: Modulation of peptidase activity by ubiquitin. Biochemistry 34(39), 12616-12623 (1995).