For immunochemical detection of citrullinated LL-37
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Citrullinated LL-37 Monoclonal Antibody (Clone 6A8)

Item No. 26739

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Antibacterial Peptide LL-37
  • Antibacterial Protein LL-37
Immunogen
Citrullinated human LL-37 peptide
Clone Designation
6A8
MW
~9 kDa
Formulation (Request formulation change)
300 µg of protein G-purified monoclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Mouse
Isotype
IgG1
Applications
ELISA, WB
Cross Reactivity
(+) Citrullinated LL-37(+) Citrullinated hCAP18(-) Native LL-37
Species Reactivity
(+) Human(+) Mouse
UniProt Accession №
P49913
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    300 µg

    $401

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    NET Formation Screening & Analysis Services
    Our experts are here to help.
    • Detection of NET formation ex vivo and screening for modulators using:
      • High-content imaging
      • Enzymatic detection
      • Citrullination/carbamylation detection
    • Experienced scientists skilled in neutrophil biology, isolation, and handling
    LEARN MORE
    Product Description

    LL-37 is a cationic α-helical peptide expressed in human bone marrow, testis, granulocytes, and gingival epithelium and a variety of immune cells.1 It has antimicrobial and antiviral activity, and protein levels of LL-37 are increased in epithelial cells, macrophages, and neutrophils following bacterial infection in vitro.1,2,3,4 LL-37 can be citrullinated by protein arginine deiminase 2 (PAD2) and PAD4, a modification that reduces its antibacterial and antiviral activities.5 Citrullinated, unlike native, LL-37 does not prevent mortality in a mouse model of D-galactosamine-sensitized endotoxic shock.6 Citrullinated LL-37 has been found in neutrophil extracellular traps (NETs), as well as the serum of patients with rheumatoid arthritis (RA) and individuals without RA.7 Cayman's Citrullinated LL-37 Monoclonal Antibody (Clone 6A8) can be used for ELISA and Western blot (WB) applications. The antibody recognizes citrullinated LL-37 at approximately 9 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Weinberg, A., Krisanaprakornkit, S., and Dale, B.A. Epithelial antimicrobial peptides: Review and significance for oral applications. Crit. Rev. Oral Biol. Med. 9(4), 399-414 (1998).

    2. Rivas-Santiago, B., Hernandez-Pando, R., Carranza, C., et al. Expression of cathelicidin LL-37 during Mycobacterium tuberculosis infection in human alveolar macrophages, monocytes, neutrophils, and epithelial cells. Infect. Immun. 76(3), 935-941 (2008).

    3. Gudmundsson, G.H., Agerberth, B., Odeberg, J., et al. The human gene FALL39 and processing of the cathelin precursor to the antibacterial peptide LL-37 in granulocytes. Eur. J. Biochem. 238(2), 325-332 (1996).

    4. Ishiyama, M., Shiga, M., Sasamoto, K., et al. A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem. Pharm. Bull. 41(6), 1118-1122 (1993).

    5. Casanova, V., Sousa, F.H., Shakamuri, P., et al. Citrullination alters the antiviral and immunomodulatory activities of the human cathelicidin LL-37 during rhinovirus infection. Front. Immunol. 11, 85 (2020).

    6. Koziel, J., Bryzek, D., Sroka, A., et al. Citrullination alters immunomodulatory function of LL-37 essential for prevention of endotoxin-induced spesis. J. Immunol. 192(11), 5363-5372 (2014).

    7. Wong, A., Bryzek, D., Dobosz, E., et al. A novel biological role for peptidyl-arginine deiminases: Citrullination of cathelicidin LL-37 controls the immunostimulatory potential of cell-free DNA. J. Immunol. 200(7), 2327-2340 (2018).