A fluorescent substrate for β-galactosidase
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Fluorescein di-β-Galactopyranoside

Item No. 26860

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3',6'-bis(β-D-galactopyranosyloxy)-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
CAS Number
17817-20-8
Synonyms
  • FDG
Molecular Formula
C32H32O15
Formula Weight
656.6
Purity
≥95%
Emission
514 nm
Excitation
490 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: soluble
SMILES
O=C(O1)C2=C(C=CC=C2)C31C4=CC=C(O[C@H]5[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O5)C=C4OC6=C3C=CC(O[C@@H]7O[C@H](CO)[C@H](O)[C@H](O)[C@H]7O)=C6
InChi Code
InChI=1S/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(41)47-32/h1-10,21-28,30-31,33-40H,11-12H2/t21-,22-,23+,24+,25+,26+,27-,28-,30-,31-/m1/s1
InChi Key
ZTOBILYWTYHOJB-WBCGDKOGSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $214

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    Product Description

    Fluorescein di-β-galactopyranoside is a fluorogenic substrate for β-galactosidase.1 Upon enzymatic cleavage by β-galactosidase, fluorescein is released and its fluorescence can be used to quantify β-galactosidase activity. Fluorescein displays excitation/emission maxima of 490/514 nm, respectively.2,1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Plovins, A., Alvarez, A.M., Ibañez, M., et al. Use of fluorescein-di-β-D-galactopyranoside (FDG) and C12-FDG as substrates for β-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells. Appl. Environ. Microbiol. 60(12), 4638-4641 (1994).

    2. Hermanson, G.T. Fluorescent Probes. Bioconjugate Techniques (2013).