A hypotensive peptide
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Proadrenomedullin (N-20) (human) (trifluoroacetate salt)

Item No. 27102

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
L-alanyl-L-arginyl-L-leucyl-L-α-aspartyl-L-valyl-L-alanyl-L-seryl-L-α-glutamyl-L-phenylalanyl-L-arginyl-L-lysyl-L-lysyl-L-tryptophyl-L-asparaginyl-L-lysyl-L-tryptophyl-L-alanyl-L-leucyl-L-seryl-L-argininamide, trifluoroacetate salt
Synonyms
  • Mid-Regional proADM
  • Mid-Regional Proadrenomedullin
  • MR-proADM
  • MR-proAM
  • proADM
  • proAM
Molecular Formula
C112H178N36O27 • XCF3COOH
Formula Weight
2460.8
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Water: 1 mg/ml
SMILES
NC([C@H](CCCNC(N)=N)NC([C@H](CO)NC([C@H](CC(C)C)NC([C@@H](NC([C@@H](NC([C@H](CCCCN)NC([C@H](CC(N)=O)NC([C@@H](NC([C@H](CCCCN)NC([C@H](CCCCN)NC([C@H](CCCNC(N)=N)NC([C@@H](NC([C@H](CCC(O)=O)NC([C@H](CO)NC([C@@H](NC([C@H](C(C)C)NC([C@@H](NC([C@H](CC(C)C)NC([C@H](CCCNC(N)=N)NC([C@@H](N[H])C)=O)=O)=O)CC(O)=O)=O)=O)C)=O)=O)=O)CC1=CC=CC=C1)=O)=O)=O)=O)CC2=CNC3=C2C=CC=C3)=O)=O)=O)CC4=CNC5=C4C=CC=C5)=O)C)=O)=O)=O)=O.OC(C(F)(F)F)=O
InChi Code
InChI=1S/C112H178N36O27.C2HF3O2/c1-57(2)46-77(102(168)147-85(56-150)107(173)132-70(90(118)156)35-23-43-125-110(119)120)139-92(158)61(8)130-100(166)80(49-64-53-128-68-30-15-13-28-66(64)68)142-97(163)73(34-19-22-42-115)136-105(171)82(51-86(117)151)144-104(170)81(50-65-54-129-69-31-16-14-29-67(65)69)143-96(162)72(33-18-21-41-114)135-94(160)71(32-17-20-40-113)134-95(161)75(37-25-45-127-112(123)124)137-103(169)79(48-63-26-11-10-12-27-63)141-99(165)76(38-39-87(152)153)138-108(174)84(55-149)146-93(159)62(9)131-109(175)89(59(5)6)148-106(172)83(52-88(154)155)145-101(167)78(47-58(3)4)140-98(164)74(133-91(157)60(7)116)36-24-44-126-111(121)122;3-2(4,5)1(6)7/h10-16,26-31,53-54,57-62,70-85,89,128-129,149-150H,17-25,32-52,55-56,113-116H2,1-9H3,(H2,117,151)(H2,118,156)(H,130,166)(H,131,175)(H,132,173)(H,133,157)(H,134,161)(H,135,160)(H,136,171)(H,137,169)(H,138,174)(H,139,158)(H,140,164)(H,141,165)(H,142,163)(H,143,162)(H,144,170)(H,145,167)(H,146,159)(H,147,168)(H,148,172)(H,152,153)(H,154,155)(H4,119,120,125)(H4,121,122,126)(H4,123,124,127);(H,6,7)/t60-,61-,62-,70-,71-,72-,73-,74-,75-,76-,77-,78-,79-,80-,81-,82-,83-,84-,85-,89-;/m0./s1
InChi Key
FGXAPPLPUSHUNN-MOWBHYGKSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 µg

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    1 mg

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    Product Description

    Proadrenomedullin (N-20) (PAMP-20) is an endogenous peptide with hypotensive activity.1 It consists of the 20 N-terminal amino acids of proadrenomedullin and is found primarily in human adrenal medulla and pheochromocytoma tissue, with small amounts present in the heart, lung, liver, spleen, pancreas, kidney, small intestine, and cerebral cortex.2 PAMP-20 is an agonist of the G protein-coupled orphan receptor MRGPRX2 that inhibits forskolin-induced cAMP accumulation in CHO cells expressing the human receptor with an EC50 value of 251 nM.3 It inhibits catecholamine secretion and sodium and calcium influx induced by the acetylcholine receptor (AChR) agonist carbachol (Item No. 14486) in bovine adrenal medullary cells in vitro (IC50s = 1.6, 2.5, and 1 μM, respectively).4 PAMP-20 induces a hypotensive response in anesthetized rats when administered at doses of 10, 20, and 50 nmol/kg by intravenous bolus injection.1 It also dose-dependently reduces systemic arterial and hindlimb perfusion pressure in anesthetized cats when injected intravenously or into the hindlimb perfusion circuit, respectively, indicating vasodilator activity.5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kitamura, K., Kangawa, K., Ishiyama, Y., et al. Identification and hypotensive activity of proadrenomedullin N-terminal 20 peptide (PAMP). FEBS Lett. 351(1), 35-37 (1994).

    2. Washimine, H., Kitamura, K., Ichiki, Y., et al. Immunoreactive proadrenomedullin N-terminal 20 peptide in human tissue, plasma and urine. Biochem. Biophys. Res. Commun. 202(2), 1081-1087 (1994).

    3. Kamohara, M., Matsuo, A., Takasaki, J., et al. Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides. Biochem. Bioph. Res. Commun. 330(4), 1146-1152 (2005).

    4. Katoh, F., Kitamura, K., Niina, H., et al. Proadrenomedullin N-terminal 20 peptide (PAMP), an endogenous anticholinergic peptide: Its exocytotic secretion and inhibition of catecholamine secretion in adrenal medulla. J. Neurochem. 64(1), 459-461 (1995).

    5. Champion, H.C., Murphy, W.A., Coy, D.H., et al. Proadrenomedullin NH2-terminal 20 peptide has direct vasodilator activity in the cat. Am. J. Physiol. 272(4 Pt. 2), R1047-R1054 (1997).