A colorimetric granzyme B and caspase-8 substrate
Related Products
Alternative(s)
42179Ac-IEPD-AFC
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Ac-IEPD-pNA (trifluoroacetate salt)

Item No. 27139

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-acetyl-L-isoleucyl-L-α-glutamyl-L-prolyl-N-(4-nitrophenyl)-L-α-asparagine, trifluoroacetate salt
Synonyms
  • Ac-Ile-Glu-Pro-Asp-p-nitroanilide
  • Caspase-8 Chromogenic Substrate
  • Granzyme B Substrate VIII
Molecular Formula
C28H38N6O11 • XCF3COOH
Formula Weight
634.6
Purity
≥95%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: 1 mg/ml
SMILES
O=C(C)N[C@H](C(N[C@H](C(N1CCC[C@H]1C(N[C@@H](CC(O)=O)C(NC2=CC=C([N+]([O-])=O)C=C2)=O)=O)=O)CCC(O)=O)=O)[C@@H](C)CC.FC(F)(C(O)=O)F
InChi Code
InChI=1S/C28H38N6O11.C2HF3O2/c1-4-15(2)24(29-16(3)35)27(42)31-19(11-12-22(36)37)28(43)33-13-5-6-21(33)26(41)32-20(14-23(38)39)25(40)30-17-7-9-18(10-8-17)34(44)45;3-2(4,5)1(6)7/h7-10,15,19-21,24H,4-6,11-14H2,1-3H3,(H,29,35)(H,30,40)(H,31,42)(H,32,41)(H,36,37)(H,38,39);(H,6,7)/t15-,19-,20-,21-,24-;/m0./s1
InChi Key
WIDZNAURMGQRCO-QIWSZQIESA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $54

    Add

    5 mg

    $172

    Add
    Product Description

    Ac-IEPD-pNA is a colorimetric substrate for granzyme B and caspase-8.1,2 Granzyme B and caspase-8 preferentially bind to and cleave the Ile-Glu-Pro-Asp (IEPD) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of granzyme B and caspase-8 activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Ewen, C., Kane, K.P., Shostak, I., et al. A novel cytotoxicity assay to evaluate antigen-specific CTL responses using a colorimetric substrate for Granzyme B. J. Immunol. Methods 276(1-2), 89-101 (2003).

    2. Almeida, S., Domingues, A., Rodrigues, L., et al. FK506 prevents mitochondrial-dependent apoptotic cell death induced by 3-nitropropionic acid in rat primary cortical cultures. Neurobiol. Dis. 17(3), 435-444 (2004).