Optineurin (C-Term) Monoclonal Antibody (Clone 8E12)

Optineurin (OPTN) is an adapter protein with roles in autophagy, vesicular trafficking, and inflammatory responses to viral and bacterial pathogens.¹ OPTN binds to ubiquitinated cargo via its ubiquitin-binding domain and targets them for autophagic degradation by recruiting LC3-expressing phagophore membranes via its LC3-interacting region. It is phosphorylated by TANK binding kinase 1 (TBK1), which increases the binding affinity of OPTN for polyubiquitinated mitochondria, promotes TBK1 activation and recruitment to the mitochondria, and permits the degradation of damaged mitochondria via PINK1/parkin-dependent mitophagy.² OPTN regulates transferrin receptor trafficking and recycling by mediating the association between the membrane trafficking protein Rab8 and its activator TBC1D17.³ Mutation of the OPTN ubiquitin binding domain reduces LPS- and poly(I:C)-induced increases in IFN-β levels in bone marrow-derived macrophages (BMDMs).⁴ OPTN knockout increases mortality and reduces plasma levels of TNF-α and IL-6 in a mouse model of E. coli-induced peritonitis.⁵ Mutations in OPTN are associated with primary open angle glaucoma, amyotrophic lateral sclerosis (ALS), Paget's disease, and Alzheimer's disease.^1,6 Cayman's Optineurin Monoclonal Antibody (Clone 8E12) can be used for immunocytochemistry applications.

WARNING This product is not for human or veterinary use.

1. Slowicka, K., Vereecke, L., and van Loo, G. Cellular functions of optineurin in health and disease. Trends Immunol. 37(9), 621-633 (2016).

2. He, L., Chen, L., and Li, L. The TBK1-OPTN axis mediates crosstalk between mitophagy and the innate immune response: A potential therapeutic target for neurodegenerative diseases. Neurosci. Bull. 33(3), 354-356 (2017).

3. Bansal, M., Swarup, G., and Balasubramanian, D. Functional analysis of optineurin and some of its disease-associated mutants. IUBMB Life 67(2), 120-128 (2015).

4. Gleason, C.E., Ordureau, A., Gourlay, R., et al. Polyubiquitin binding to optineurin is required for optimal activation of TANK-binding kinase 1 and production of interferon β. The Journal of Biological Chemisty 286(41), 35663-35674 (2011).

5. Chew, T.S., O'Shea, N.R., Sewell, G.W., et al. Optineurin deficiency in mice contributes to impaired cytokine secretion and neutrophil recruitment in bacteria-driven colitis. Dis. Model Mech. 8(8), 817-829 (2015).

6. Toth, R.P., and Atkin, J.D. Dysfunction of optineurin in amyotrophic lateral sclerosis and glaucoma. Front. Immunol. 9, 1017 (2018).