A radical cation used to assess antioxidant capacity
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ABTS (ammonium salt)

Item No. 27317

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2,2'-(1,2-hydrazinediylidene)bis[3-ethyl-2,3-dihydro-6-benzothiazolesulfonic acid, diammonium salt
CAS Number
30931-67-0
Molecular Formula
C18H16N4O6S4 • 2NH4
Formula Weight
548.7
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 10 mg/mlPBS (pH 7.2): 3 mg/ml
λmax
224, 346 nm
SMILES
[O-]S(C1=CC=C(N(CC)/C(S2)=N/N=C3SC(C=C(S([O-])(=O)=O)C=C4)=C4N\3CC)C2=C1)(=O)=O.[NH4+].[NH4+]
InChi Code
InChI=1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
InChi Key
OHDRQQURAXLVGJ-AXMZSLBLSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

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    5 g

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    10 g

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    Product Description

    ABTS is a radical cation and a substrate of peroxidases, including horseradish peroxidase (HRP).1 It has commonly been used to assess antioxidant capacity in the Trolox equivalent antioxidant capacity (TEAC) assay.2,3 It has a blue color in the presence of sodium persulfate or metmyoglobin but decolorizes upon incubation with antioxidants, and the antioxidant capacity can be determined spectrophotometrically. ABTS has also been used as an enzyme substrate in ELISAs.4,5 Upon incubation with a peroxidase, it produces a soluble green product that can be quantified by colorimetric detection at 405 nm.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bhattarai, H.D., Paudel, B., Lee, H.S., et al. Antioxidant activity of Sanionia uncinata, a polar moss species from King George Island, Antarctica. Phytother. Res. 22(12), 1635-1639 (2008).

    2. Huang, D., Ou, B., and Prior, R.L. The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 53(6), 1841-1856 (2005).

    3. Walker, R.B., and Everette, J.D. Comparative reaction rates of various antioxidants with ABTS radical cation. J. Agric. Food Chem. 57(4), 1156-1161 (2009).

    4. Reyna-Bello, A., Eleizalde, M.C., and Silva, A.M. Assessment of chromogen suitability in ELISA for the detection of anaplasmosis and trypanosomosis. J. Immunoassay Immunochem. 28(1), 1-11 (2007).

    5. Su, Y.C., and Wong, A.C. Detection of staphylococcal enterotoxin H by an enzyme-linked immunosorbent assay. J. Food Prot. 59(3), 327-330 (1996).