For immunochemical detection of GPR18
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GPR18 (C-Term) Polyclonal Antibody

Item No. 27342

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • N-Arachidonyl Glycine Receptor
  • GPCRW
  • G Protein-Coupled Receptor 18
  • NAGly Receptor
Immunogen
Synthetic peptide from the C-terminal region of human GPR18
Formulation (Request formulation change)
500 µl of peptide affinity-purified antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
ELISA and IHC
Species Reactivity
(+) Human; other species not tested
UniProt Accession №
Q14330
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    500 µl

    $468

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    Product Description

    GPR18 is a G protein-coupled receptor (GPCR) that is expressed in lymphoid tissues, lung, brain, testis, and ovaries.1 It is coupled to the Gai/o and Gaq/11 transduction pathways and is activated by the polyunsaturated fatty acid metabolite resolvin D2 (Item No. 10007279) and the endocannabinoid arachidonoyl glycine (NAGly; Item No. 90051) in a context-dependent manner.1,2 Resolvin D2-induced activation of GPR18 reduces inflammatory pain induced by formalin, carrageenan, and complete Freund's adjuvant (CFA) in rats and reduces production of pro-inflammatory cytokines in mouse models of cecal ligation and puncture-induced sepsis, ocular infections, and ischemic injury.3,4 NAGly-induced activation of GPR18 lowers intraocular pressure (IOP) in mice in a fatty acid amide hydrolase-dependent manner.5 GPR18 is also overexpressed and constitutively active in patient-derived metastatic melanoma tumor samples.6 Cayman's GPR18 (C-Term) Polyclonal Antibody can be used for ELISA and immunohistochemistry (IHC) applications. The antibody recognizes the C-terminal region of GPR18 from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Morales, P., and Reggio, P.H. An update on non-CB1, non-CB2 cannabinoid related G-protein-coupled receptors. Cannabis Cannabinoid Res. 2(1), 265-273 (2017).

    2. Mahardhika, A.B., Załuski, M., Schoeder, C.T., et al. Potent, selective agonists for the cannabinoid-like orphan G protein-coupled receptor GPR18: A promising drug target for cancer and immunity. J. Med. Chem. 67(12), 9896-9926 (2024).

    3. Zhang, L.-Y., Liu, Z.-H., Zhu, Q., et al. Resolvin D2 relieving radicular pain is associated with regulation of inflammatory mediators, Akt/GSK-3β signal pathway and GPR18. Neurochem. Res. 43(12), 2384-2392 (2018).

    4. Zhang, M.J., Sansbury, B.E., Hellmann, J., et al. Resolvin D2 enhances post-ischemic revascularization while resolving inflammation. Circulation 134(9), 666-680 (2016).

    5. Miller, S., Leishman, E., Oehler, O., et al. Evidence for a GPR18 role in diurnal regulation of intraocular pressure. Invest. Ophthalmol. Vis. Sci. 57(14), 6419-6426 (2016).

    6. Qin, Y., Verdegaal, E.M.E., Siderius, M., et al. Quantitative expression profiling of G-protein-coupled receptors (GPCRs) in metastatic melanoma: The constitutively active orphan GPCR GPR18 as novel drug target. Pigment Cell Melanoma Res. 24(1), 207-218 (2011).