A colorimetric reagent for the detection of lipid peroxidation
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2-Thiobarbituric Acid

Item No. 27367

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
dihydro-2-thioxo-4,6(1H,5H)-pyrimidinedione
CAS Number
504-17-6
Synonyms
  • 4,6-Dihydroxy-2-mercaptopyrimidine
  • 2-Mercaptobarbituric Acid
  • NSC 4733
Molecular Formula
C4H4N2O2S
Formula Weight
144.1
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 12 mg/mlDMSO: 12 mg/mlEthanol: 0.2 mg/mlPBS (pH 7.2): 0.5 mg/ml
λmax
212, 284 nm
SMILES
O=C(CC(N1)=O)NC1=S
InChi Code
InChI=1S/C4H4N2O2S/c7-2-1-3(8)6-4(9)5-2/h1H2,(H2,5,6,7,8,9)
InChi Key
RVBUGGBMJDPOST-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    25 g

    $41

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    50 g

    $75

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    100 g

    $142

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    250 g

    $312

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    Product Description

    2-Thiobarbituric acid is a colorimetric reagent commonly used in the detection of malondialdehyde (MDA), a marker of lipid peroxidation.1,2 2-Thiobarbituric acid forms a complex with MDA that can be quantified by colorimetric detection at 532 nm as a measure of lipid peroxidation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Knight, J.A., Pieper, R.K., and McClellan, L. Specificity of the thiobarbituric acid reaction: Its use in studies of lipid peroxidation. Clin. Chem. 34(12), 2433-2438 (1988).

    2. Reitznerová, A., Šuleková, M., Nagy, J., et al. Lipid peroxidation process in meat and meat products: A comparison study of malondialdehyde determination between modified 2-thiobarbituric acid spectrophotometric method and reverse-phase high-performance liquid chromatography. Molecules 22(11), E1988 (2017).