A reactive probe for derivatization of primary amines
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Dansyl Chloride

Item No. 27458

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-(dimethylamino)-1-naphthalenesulfonyl chloride
CAS Number
605-65-2
Synonyms
  • DNSCl
  • NSC 83616
Molecular Formula
C12H12ClNO2S
Formula Weight
269.7
Purity
≥95%
Emission
535 nm, in acetone
Excitation
340 nm, in acetone
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 10 mg/mlDMSO:PBS (pH 7.2) (1:40): 0.02 mg/mlEthanol: 1 mg/ml
λmax
210, 265, 369 nm
SMILES
CN(C)C1=CC=CC2=C1C=CC=C2S(Cl)(=O)=O
InChi Code
InChI=1S/C12H12ClNO2S/c1-14(2)11-7-3-6-10-9(11)5-4-8-12(10)17(13,15)16/h3-8H,1-2H3
InChi Key
XPDXVDYUQZHFPV-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

    $41

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    5 g

    $140

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    10 g

    $255

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    25 g

    $534

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    Product Description

    Dansyl chloride is a reactive probe for the derivatization of primary amines, including those on amino acids, peptides, and polyamines, for detection by HPLC.1,2,3 It has also been used for the derivatization of compounds containing phenol groups, such as steroids, for detection by MS/MS.4 Dansyl chloride is a fluorescent probe for proteins and enzymes.5 It displays excitation/emission maxima of 340/535 nm, respectively, in acetone.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Walker, J.M. The dansyl method for identifying N-terminal amino acids. Methods in molecular biology: Basic protein and peptide protocols 32, 321-328 (1994).

    2. Takeuchi, T. HPLC of amino acids as dansyl and dabsyl derivatives. J. Chromatogr. Lib. 70, 229-241 (2005).

    3. Hunter, K.J. A dansyl chloride-HPLC method for the determination of polyamines. Methods in molecular biology: Polyamine protocols 79, 119-123 (1998).

    4. Santa, T. Derivatization reagents in liquid chromatography/electrospray ionization tandem mass spectrometry. Biomed. Chromatogr. 25(1-2), 1-10 (2011).

    5. Chen, R.F., and Scott, C.H. Atlas of fluorescence spectra and lifetimes of dyes attached to protein. Anal. Lett. 18(4), 393-421 (1985).