A colorimetric thrombin substrate
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N-(p-Tosyl)-GPR-pNA (acetate)

Item No. 27568

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[(4-methylphenyl)sulfonyl]glycyl-L-prolyl-N-(4-nitrophenyl)-L-argininamide, monoacetate
CAS Number
86890-95-1
Synonyms
  • N-(p-Tosyl)-Gly-Pro-Arg-p-Nitroanilide
  • Tos-Gly-Pro-Arg-pNA
Molecular Formula
C26H34N8O7S • C2H4O2
Formula Weight
662.7
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 3 mg/mlPBS (pH 7.2): 3 mg/ml
λmax
225, 314 nm
SMILES
CC1=CC=C(S(NCC(N2CCC[C@H]2C(N[C@@H](CCCNC(N)=N)C(NC3=CC=C([N+]([O-])=O)C=C3)=O)=O)=O)(=O)=O)C=C1.OC(C)=O
InChi Code
InChI=1S/C26H34N8O7S.C2H4O2/c1-17-6-12-20(13-7-17)42(40,41)30-16-23(35)33-15-3-5-22(33)25(37)32-21(4-2-14-29-26(27)28)24(36)31-18-8-10-19(11-9-18)34(38)39;1-2(3)4/h6-13,21-22,30H,2-5,14-16H2,1H3,(H,31,36)(H,32,37)(H4,27,28,29);1H3,(H,3,4)/t21-,22-;/m0./s1
InChi Key
NGXDRWVTRQRSED-VROPFNGYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    25 mg

    $175

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    50 mg

    $332

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    100 mg

    $592

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    Product Description

    N-(p-Tosyl)-GPR-pNA is a colorimetric substrate for thrombin.1 Thrombin preferentially binds to and cleaves the Gly-Pro-Arg (GPR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of thrombin activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Abildgaard, U., Lie, M., and Ødegȧrd, O.R. Antithrombin (heparin cofactor) assay with "new" chromogenic substrates (S-2238 and Chromozym TH). Thromb. Res. 11(4), 549-553 (1977).